MECHANISMS OF MICROBIAL IRON TRANSPORT

微生物铁运输机制

• 批准号: 3124585
• 负责人: THOMAS F. EMERY
• 金额: $ 9.37万
• 依托单位: UTAH STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-09-01 至 1995-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3124585
• 关键词: acidity /alkalinity; biological models; biological transport; chelating agents; cytoplasm; ionophores; microorganism growth; microorganism metabolism; mitochondria; protein structure; siderophores; transferrin; transport proteins; vesicle /vacuole

The least understood aspect of mammalian iron metabolism is the fate of the metal in the cell cytosol. Transferrin is taken into the cell and held in specific compartments called CURL. The exact mechanism by which the iron is dissociated from the transferrin, the chemical form of iron that enters the cytosol. Furthermore, there is evidence that alternate reductive mechanisms of iron transport are sometimes utilized for cellular uptake, but such processes have been little investigated. A simple model system is lacking. The protozoan, Tetrahymena pyriformis, will be utilized as a model of animal iron transport. This organism, like higher animals, packages ingested iron in vacuoles from which the metal must pass through the cytosol and into mitochondria. A procedure for the rapid purification of these vacuoles will be established. Vacuoles will be isolated containing different forms of iron ranging from microbial siderophores to transferrin. The role of pH, cellular reducing agents, and hydrolytic enzymes in iron solubilization and export into the cytosol will be investigated. The chemical form of iron in the cytosol will be determined and utilized in studying iron uptake mechanisms by the mitochondria. In addition to providing a model for mammalian iron transport, the knowledge obtained may be relevant to the control of infectious diseases caused by protozoa.

哺乳动物铁代谢的最不知情的方面是 金属中的细胞胞质中。 转铁蛋白被带入电池并将 特定的隔间称为卷曲。 铁的确切机制 与转铁蛋白分离，铁的化学形式进入 细胞质。 此外，有证据表明替代还原性 铁运输机制有时用于细胞摄取， 但是这样的过程几乎没有调查。 一个简单的模型系统是 缺乏。 原生动物，四膜吡虫菌素将被用作 动物铁运输。 这种有机体，像高等动物一样，包装 金属必须通过的液泡中摄入铁 细胞质并进入线粒体。 快速净化的程序 这些液泡将建立。 液泡将被隔离 从微生物铁载体到转铁蛋白的不同形式的铁。 pH，细胞还原剂和水解酶在铁中的作用 将研究溶解并导出到细胞质中。 这 将确定并利用化学形式的铁中铁 通过线粒体研究铁摄取机制。 此外 提供了哺乳动物铁运输的模型，获得的知识可能 与原生动物引起的传染病的控制有关。