GENETICS OF LAMBDA PROPHAGE--BIOTIN OPERON

Lambda原噬菌体--生物素操纵子的遗传学

• 批准号: 3124423
• 负责人: ALLAN M CAMPBELL
• 金额: $ 25.81万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-06-01 至 1993-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3124423
• 关键词: bacteriophage lambda; prophages; virus genetics

The long term goals are to understand how viruses interact with chromosomes and how genes for biosynthesis of proteins needed in small amounts are regulated. The health relatedness of these studies on prokaryotic model systems lies in the elucidation of basic mechanisms and in the perspective shed on natural biology of disease-causing viruses by the common features of bacterial and animal virology. The DNA of bacteriophage lambda upstream of the integrase operon will be examined for effects on integration and excision of two elements: a terminator-like sequence tI' that abuts the pI promoter and an open reading frame (ORF-55) upstream from it. Phage constructed to eliminate tI' will be examined for the effect of cII protein in the presence of transcription from pL, to test the hypothesis that tI' functions in the initial transition from the uncommitted to the lysogenic state. The ORF-55 gene expressed from a plasmid will be tested for complementation of the excisionase defect of the lambda crg phage, which has an IS2 insertion in ORF-55. Lambda crg prophages in inverted orientation will be tested for the ability to generate duplications by recombination with the defective gsr' prophage, and the defective prophages of E. coli strains of the ECOR collection will be compared. The properties of purified E. coli biotin sulfoxide reductase and the molecular basis for a newly evolved BDS reductase activity will be determined.

长期目标是了解病毒如何与 染色体以及如何在蛋白质中需要生物合成的基因 少量监管。 这些与健康相关性 核模型系统的研究在于阐明 基本机制和自然生物学的视角 通过细菌的共同特征引起疾病的病毒 和动物病毒学。 噬菌体lambda上游的DNA 将检查集成酶操纵子的影响是否 两个元素的集成和切除：类似终结者 将PI启动子和开放式阅读框架毗邻的序列 （ORF-55）从中上游。 伪造以消除ti'的噬菌体 将在存在的情况下检查CII蛋白的作用 从PL转录，以检验Ti在功能中的假设 从未承诺的溶酶因状态的初始过渡。 从质粒表达的ORF-55基因将进行测试 Lambda CRG的切除酶缺陷的互补 噬菌体，在ORF-55中插入IS2。 Lambda Crg 将测试倒置方向的预言的能力 通过与缺陷的GSR重组产生重复 预言和大肠杆菌菌株的缺陷预言 将比较ECOR收集。 纯化E的特性。 大肠杆菌生物素亚磺氧化物还原酶和新近的分子基础 将确定进化的BDS还原酶活性。