STRUCTURAL STUDIES ON RNA-CONTAINING VIRAL GENOMES

含RNA病毒基因组的结构研究

• 批准号: 3126124
• 负责人: ANN B JACOBSON
• 金额: $ 11.36万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-09-01 至 1986-09-21
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3126124
• 关键词: HeLa cells; Polyomavirus; chromosome deletion; computer data analysis; electron microscopy; genetic mapping; genome; messenger RNA; nucleic acid sequence; ribosomes; tissue /cell culture; virus genetics

Combined electron microscopic and computer studies will be used to obtain information on the type of structures which are involved in the folding of single-stranded RNAs from the bacteriophage MS2, poliovirus and T7 mRNA. The work has been facilitated by the recent development of a high speed computer program for predicting loops from sequence data on large molecules. Improved electron microscopic mapping data will be obtained by hybridizing restriction fragments of cloned MS2 DNA to MS2 RNA. The hybrid molecules will be examined for the loss of loop structures in the electron microscope.

相结合的电子显微镜和计算机研究将用于获得 有关折叠的结构类型的信息 来自噬菌体MS2，脊髓灰质炎病毒和T7 mRNA的单链RNA。 这 高速计算机的最新开发使工作促进了工作 从大分子上的序列数据中预测循环的程序。 改进 电子微观映射数据将通过杂交限制获得 克隆的MS2 DNA到MS2 RNA的片段。 将检查混合分子 为了损失电子显微镜中的环结构。

项目成果

Effect of spermidine on the conformation of bacteriophage MS2 RNA. Electron microscopy and computer modeling.

亚精胺对噬菌体 MS2 RNA 构象的影响。

• DOI: 10.1016/0022-2836(85)90424-3
• 发表时间: 1985
• 期刊: Journal of molecular biology
• 影响因子: 5.6
• 作者: Jacobson,AB;Kumar,H;Zuker,M
• 通讯作者: Zuker,M