STUDIES OF THE STRUCTURES OF FCY RECEPTORS

FCY受体结构的研究

基本信息

批准号：
3126934
负责人：
ANTHONY KULCZYCKI
金额：
$ 14.15万
依托单位：
WASHINGTON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1980
资助国家：
美国
起止时间：
1980-07-01 至 1987-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3126934
关键词：
antibody specificity basophils gel electrophoresis human tissue humoral immunity immunoglobulin E immunoglobulin structure immunoregulation lymphocyte macrophage tissue /cell culture

项目摘要

The aims of this grant proposal are: (1) To further characterize the structurally unique FcGamma receptors isolated from human monocytes, B lymphocytes, and non-B lymphocytes. Purified cell populations will be labeled with radioactive isotypes, and radiolabeled FcGamma receptors will be isolated by repetitive chromatography. Labeled FcGamma receptors will be analyzed by SDS-polyacrylamide gel electrophoresis. Tryptic peptide maps and amino acid sequences of the unique receptors and their fragments will be examined for homology. (2) To isolate and characterize the FcGamma receptors of other human leukocyte populations e.g. eosinophils, human cell lines, leukemia cells using similar procedures. (3) To prepare monoclonal antibodies against each of the distinct human FcGamma receptors.F(ab) and F(ab)'s fragments of monoclonal proteins will be carefully characterized to rigorously document anti-receptor activity. (4) To study interactions between the distinct human FcGamma receptors and immunoglobulins which differ by subclass, class, species of origin, or state of aggregation. (5) To determine whether human FcGamma receptors have multiple components. Biosynthetically radiolabeled receptors and chemically crosslinked receptors will be examined for evidence of, and properties of, possible associated molecules. (6) If FcGamma receptors are phosphoproteins, to examine whether receptors are selectively phosphorylated (or dephosphorylated) during non-specific or immunologic cell activation. (Disciplines involved are immunology, protein chemistry, and cell biology.) These aims will further our long term objectives: (a) Learning about the distributions, structures, and functions of the structurally unique human FcGamma receptors, their interactions with immunoglobulins, and their mechanisms of action. (b) Determining whether FcGamma receptors may be involved in complications of leukemia and whether detection of unique FcGamma receptors may be useful in lymphoma classification and treatment. (c) Understanding mechanisms of immunodeficiency and other immunologic diseases.

该赠款提案的目的是：（1）进一步表征从人类单核细胞分离出的结构独特的Fcgamma受体B，B 淋巴细胞和非B淋巴细胞。纯化的细胞种群将是标有放射性同种型，放射标记的FCGAMMA受体将通过重复色谱分离。标记的fcgamma受体将通过SDS-聚丙烯酰胺凝胶电泳分析。胰蛋白酶肽独特受体及其片段的地图和氨基酸序列将检查同源性。（2）隔离和表征fcgamma 其他人类白细胞种群的受体，例如嗜酸性粒细胞，人类细胞线，白血病细胞使用相似的程序。（3）准备单克隆针对每个独特的人fcgamma受体的抗体。F（AB）和 F（AB）的单克隆蛋白片段将仔细地表征为严格记录抗受体活动。（4）研究互动在独特的人fcgamma受体和免疫球蛋白之间通过子类，阶级，起源物种或聚集状态不同。（5）确定人Fcgamma受体是否具有多个成分。生物合成的放射性标记受体和化学交联的受体将检查受体是否有可能的证据和性质相关分子。（6）如果Fcgamma受体是磷蛋白，则检查受体是否被选择性地磷酸化（或在非特异性或免疫细胞激活期间去磷酸化）。（涉及的学科是免疫学，蛋白质化学和细胞生物学。）这些目标将进一步我们的长期目标：（a）学习关于结构上的分布，结构和功能独特的人类Fcgamma受体，它们与免疫球蛋白的相互作用，及其行动机制。（b）确定fcgamma受体是否可能参与白血病的并发症以及是否检测独特的FCGAMMA受体可能在淋巴瘤分类和治疗。（c）理解免疫缺陷和其他的机制免疫疾病。