STREPTOCOCCUS PLASMIDS: MOLECULAR GENETIC ANALYSIS

链球菌质粒：分子遗传学分析

• 批准号: 3126292
• 负责人: VICKERS BURDETT
• 金额: $ 14.68万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-01-01 至 1987-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3126292
• 关键词: DNA; Streptococcus; communicable disease transmission; electron microscopy; gel electrophoresis; genetic manipulation; genetic strain; mixed tissue /cell culture; molecular weight; mutant; plasmids; virus genetics

Research activities in this laboratory are directed towards an understanding of the biology and evolution of extrachromosomal (plasmid) elements in Streptococcus ssp. During the current year, we have identified several different types of tetracycline resistant plasmids from group B Streptococcus. Several of these reside on small non-conjugative plasmids which are capable of transforming S. sanguis Challis. For this reason, they are potentially useful as cloning vehicles in this system. We have cloned two tetracycline determinants in the E. coli system using ColEl-amp as the cloning vehicle. These chimeras have been used to generate a large series of deletions which have given a precise definition of the tetracycline resistance region. In addition, we have used the chimeras to make probes for southern hybridization experiments. These experiments have also led to a better understanding of the tetracycline resistance determinants.

该实验室的研究活动是针对理解的 肉毒外（质粒）元素的生物学和进化 链球菌SSP。 在当年，我们已经确定了几个 B组链球菌的不同类型的四环素抗性质粒。 其中几个驻留在能够 改变S. Sanguis Challis。 因此，它们可能有用 作为该系统中的克隆车辆。 我们使用大肠杆菌系统中的两个四环素决定因素克隆了 Colel-Amp作为克隆车辆。 这些嵌合体已用于生成 一系列删除，给出了确切的定义 四环素抵抗区域。 此外，我们还使用嵌合体制作 南部杂交实验的探针。 这些实验也引起了 更好地理解四环素抗性决定因素。