ADENYLYL CYCLASES IN TRANSFORMATION OF MAMMARY EPITHELIA

腺苷酸环化酶在乳腺上皮细胞转化中的作用

• 批准号: 2709530
• 负责人: PRAHLAD T RAM
• 金额: $ 2.5万
• 依托单位: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-01-05 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2709530
• 关键词: Animalia; adenylate cyclase; breast neoplasms; carcinogenesis; cell line; cell proliferation; cyclic AMP; enzyme activity; enzyme mechanism; gene expression; mammary epithelium; neoplastic cell; neoplastic transformation; phenotype; polymerase chain reaction; protein isoforms; protein tyrosine kinase; tissue /cell culture; transfection

Breast cancer is a disease process that impacts the life of thousands of women every year. Advances in understanding the molecular basis of the disease has been of tremendous help in the fight against breast cancer. From all the data that has been generated thus far we know that increasing the cAMP levels in breast tumor cells leads to inhibition of tumor cell proliferation. Further, cAMP levels have also been demonstrated to have an affect on multidrug resistant tumors, whereby increasing cAMP levels causes the resistant cells to be suceptable to chemotherapeutic drugs. The aim of this proposal is to understand the role of adenylyl cyclases in the regulation of transformed mamary epithelia. In addition it will also explore the potential therupuric benefit of expressing specific adenylyl cyclase isoforms in transformed and multidrug resistant cells. The proposal will also examine the molecular and biochemical aspects of the signaling pathways, in mammary epithelia, to understand the mechanisms by which interactions between the signaling pathways can be used to regulate transformation.

乳腺癌是一种影响数千人生命的疾病过程 每年都有女性。 理解分子基础的进展 这种疾病对于对抗乳腺癌有巨大的帮助 癌症。 从迄今为止生成的所有数据中我们知道 增加乳腺肿瘤细胞中的 cAMP 水平会抑制 肿瘤细胞增殖。 此外，cAMP 水平也已 已被证明对多重耐药肿瘤有影响，其中 增加 cAMP 水平会导致耐药细胞更容易受到 化疗药物。 该提案的目的是了解 腺苷酸环化酶在转化乳腺调控中的作用 上皮细胞。 此外，它还将探索潜在的治疗方法 在转化中表达特定腺苷酸环化酶亚型的好处 和多重耐药细胞。 该提案还将审查 信号通路的分子和生化方面，在乳腺中 上皮细胞，了解细胞之间相互作用的机制 信号通路可用于调节转化。