ETHANOL EFFECTS ON HIPPOCAMPAL NEURONAL ACTIVITY

乙醇对海马神经元活动的影响

• 批准号: 3111366
• 负责人: LARRY P GONZALEZ
• 金额: $ 10.21万
• 依托单位: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-07-01 至 1991-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3111366
• 关键词: alcoholic beverage consumption; alcoholism /alcohol abuse; anesthetics; anticholinergic agent; cholinergic agents; drug adverse effect; drug interactions; drug metabolism; drug tolerance; drug withdrawal; electrical potential; electrodes; electrophysiology; ethanol; evoked potentials; gas chromatography; hippocampus; laboratory rat; neurons; neurophysiology; nicotinic receptors; statistics /biometry

Exposure to either acute or chronic ethanol alters the activity of hippocampal cholinergic neurons. Nuerochemical and electrophysiological measures of cellular activity are responsive to ethanol, and several effects of ethanol can be blocked or potentiated by drugs which modify central cholinergic activity. Further, long-lasting pathological changes have been observed in the hippocampus of animals chronically treated with ethanol for extended periods of time. In order to determine the direct effects of ethanol on hippocampal neurons, the proposed studies will examine the electrophysiological activity of an identified population of hippocampal cells, and will study the effects of acute and chronic ethanol exposure. This will be done by characterizing the spontaneous and evoked activity of cells in the hippocampal CA3 field and determining the effects of acute, systemic ethanol administration, both before and after chronic ethanol exposure, in an acute animal preparation. Local application of receptor agonists and antagonists will be performed to further characterize the observed population of cells, and will permit a determination of the effects of ethanol treatment on neuronal responsiveness. Various aspects of the development and dissipation of tolerance, of progressive changes in neuronal activity with continuing ethanol exposure, of changes in activity during withdrawal from chronic ethanol, and of the presence of residual, long-lasting effects of chronic ethanol will also be investigated. These studies should provide a greater understanding of the central effects of ethanol on a defined cell population and of the specific neuronal alterations which accompany chronic ethanol exposure and the development of tolerance to ethanol.

暴露于急性或慢性乙醇会改变活性 海马胆碱能神经元。 Nuerochemical和 细胞活性的电生理测量是反应性的 到乙醇，乙醇的几种作用可以阻塞或 通过修饰中央胆碱能活性的药物增强。 此外，在 用乙醇长期治疗动物的海马 长时间。 为了确定直接 乙醇对海马神经元的影响，拟议的研究 将检查已鉴定的电生理活性 海马细胞的种群，并将研究 急性和慢性乙醇暴露。 这将由 表征细胞在 海马CA3场并确定急性的影响 慢性前后的全身乙醇给药 在急性动物制备中，乙醇暴露。 当地的 将执行受体激动剂和拮抗剂的应用 进一步表征观察到的细胞群体，并将 允许确定乙醇处理对 神经元反应能力。 发展的各个方面 公差的耗散，神经元的进行性变化 持续乙醇暴露的活动，活动变化 在退出慢性乙醇和存在期间 慢性乙醇的残留，持久作用也将是 调查。 这些研究应提供更大的 了解乙醇对定义细胞的中心作用 人口和特定神经元改变 伴随慢性乙醇暴露和发展 对乙醇的耐受性。