CORE--CLINICAL VIROLOGY

核心——临床病毒学

• 批准号: 3791377
• 负责人: JAMES R CARLSON
• 金额: --
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3791377
• 关键词: AIDS /HIV test; T lymphocyte; antiviral antibody; biohazard control; biomedical facility; histochemistry /cytochemistry; human T cell lymphotropic virus type 1; human immunodeficiency virus 1; human immunodeficiency virus 2; immunochemistry; serology /serodiagnosis; simian immunodeficiency virus; virus antigen; virus classification

The CLINICAL VIROLOGY CORE serves as a central laboratory for clinical and basic investigators in the California University wide Task Force on AIDS, the UC Davis Medical Center in Sacramento, the PRIMATE CENTER CORE, and the MOLECULAR VIROLOGY CORE. The CLINICAL VIROLOGY CORE will provide the equipment, materials and expertise to support projects that require laboratory diagnostic tests for retrovirus infections or retrovirus cell culture under biocontainment level 3 (BL 3) conditions. These projects involve several retroviruses including HIV-l, HIV-2, HTLV-l, STLV-I, SIVmac and SIVsm. Diagnostic methods include primary virus isolation and culture with adaptation of isolates to continuous cell lines, serologies for the detection of antiviral antibodies or viral antigens, eg., HIV p24, immunohistochemical methods, and other indicators of disease progression including beta-2-microglobulin and T-cell levels. Retrovirus cell culture methods are applied to projects that require large scale production and purification of retrovirus antigens, analysis of inactivation or neutralization of retrovirus infectivity, and other in vitro retrovirology experiments. The CLINICAL VIROLOGY CORE supports a central role in the collection, processing, accessioning, and triage of specimens from both human and simian sources.

临床病毒学核心作为中心实验室 加州大学的临床和基础研究人员 艾滋病工作组，位于萨克拉门托的加州大学戴维斯分校医学中心， 灵长类动物中心核心和分子病毒学核心。 临床 VIROLOGY CORE 将提供设备、材料和专业知识 支持需要实验室诊断测试的项目 逆转录病毒感染或逆转录病毒细胞培养 生物防护等级 3 (BL 3) 条件。 这些项目涉及 多种逆转录病毒，包括 HIV-1、HIV-2、HTLV-1、STLV-I、SIVmac 和 SIVsm。 诊断方法包括原发性病毒分离和 使分离株适应连续细胞系的培养， 用于检测抗病毒抗体或病毒的血清学 抗原，例如 HIV p24、免疫组织化学方法和其他 疾病进展指标，包括 β-2-微球蛋白 和T细胞水平。 逆转录病毒细胞培养方法应用于 需要大规模生产和纯化的项目 逆转录病毒抗原，失活或中和分析 逆转录病毒感染性和其他体外逆转录病毒学 实验。 临床病毒学核心支持核心作用 在收集、处理、加入和分类中 来自人类和猿猴的标本。