LIPOPROTEIN SUBCLASSES STRUCTURE, ORIGIN AND METABOLISM

脂蛋白亚类结构、起源和代谢

• 批准号: 3097645
• 负责人: ALEXANDER V NICHOLS
• 金额: $ 201.57万
• 依托单位: UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB
• 依托单位国家: 美国
• 财政年份: 1976
• 资助国家: 美国
• 起止时间: 1976-04-01 至 1991-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3097645
• 关键词: atherosclerosis; blood lipoprotein; blood lipoprotein metabolism; chemical structure function; clinical biomedical equipment; lipid structure; lipoprotein disorder; molecular pathology

This research program continues to focus on serum lipoproteins considered as biochemical entities of significance in the etiology of atherosclerosis, lipid disorders and other diseases. Understanding such relationships requires the elucidation of the chemical, physical, and functional characteristics of the lipoproteins. Our research therefore emphasizes detailed characterization, as well as quantification, of serum lipoproteins in terms of classes and subclasses, distributions, interrelationships and origins. Our investigations of the lipoproteins will address a variety of problems and employ a broad scope of experimental techniques and skills. Among the principal tools that we expect to develop and apply to fundamental biophysical and biomedical problems are: analytic ultracentrifugation, electrophoresis, electron microscopy, chromatography, enzymatic methods and computer technology. A new apolipoprotein core unit will be directed toward determination of specific apolipoproteins. Lipoprotein structure and function will be examined in native and partially degraded particles and in physical or enzymatically reassembled model structures. Transformation in lipoprotein distributions will be studied in vitro as well as in vivo; the latter as a function of diet, drugs and disease. Human cord blood will be analyzed for information and insights into the genesis of lipoproteins. A major methodologic effort will be concerned with detection and minimization of potential artifacts and degradation arising from lipoprotein isolation and analysis. The unique methodologic resources of this Program Project will be available for outside collaborations on specialized or unique biomedical problems. The interactive design of developing methodology with application to basic and clinical research problems remains a major feature of our Program Project.

该研究计划继续专注于被认为是的血清脂蛋白 在动脉粥样硬化，脂质病因学中具有重要意义的生化实体 疾病和其他疾病。 了解这种关系需要 阐明化学，物理和功能特征的 脂蛋白。 因此，我们的研究强调了详细的特征， 以及定量，在类中的血清脂蛋白和 子类，分布，相互关系和起源。 我们的调查 脂蛋白的含量将解决各种问题，并采用广泛的范围 实验技术和技能。 我们期望的主要工具 开发和应用于基本的生物物理和生物医学问题是： 分析性超速离心，电泳，电子显微镜， 色谱，酶方法和计算机技术。 一种新的载脂蛋白 核心单位将针对确定特定的载脂蛋白。 脂蛋白结构和功能将在本机和部分检查 降解的颗粒以及物理或酶上的重新组装模型 结构。 将研究脂蛋白分布的转化 体外和体内；后者是饮食，药物和疾病的函数。 将分析人类的脐带血，以获取有关创世纪的信息和见解 脂蛋白。 主要的方法论工作将与检测有关 以及脂蛋白引起的潜在伪影和降解的最小化 隔离和分析。 该程序的独特方法论资源 项目将用于专业或独特的外部合作 生物医学问题。 开发方法的交互式设计与 在基本和临床研究问题上的应用仍然是 我们的计划项目。