SYNAPTIC RELATIONSHIPS OF CHEMORECEPTOR NEURONS

化学感受器神经元的突触关系

• 批准号: 3854029
• 负责人: THOMAS E FINGER
• 金额: --
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3854029
• 关键词: afferent nerve; antigens; catfish; cell adhesion; cell differentiation; central nervous system; chemoreceptors; electron microscopy; hamsters; hippocampus; histochemistry /cytochemistry; immunochemistry; laboratory mouse; laboratory rat; limbic system; medulla oblongata; membrane proteins; neural degeneration; neural transmission; neuroanatomy; neurogenesis; neurotransmitters; olfactory nerve; radiotracer; synapses; taste; taste buds; taste threshold

Turnover of the chemosensory cells of the gustatory and olfactory system necessitates substantial neuronal growth and remodelling of synaptic connections in the peripheral and central nervous systems. Experiments within this project are designed to test the degree of neuronal growth and plasticity in these systems through the use of immunocytochemical probes which are directed against neuronal growth-related antigens. These experiments will examine primary chemosensory nerves and the receptor cells of the taste buds for evidence of immunoreactivity to antibodies directed against the neuronal membrane proteins 5B4 and GAP- 43, both of which are present in high levels in growing neurons but are reduced in synaptically stable neurons. Taste bud cells themselves will be tested for the presence of GAP-43 since some taste cells express 5B4 immunoreactivity. Tritiated thymidine techniques will be utilized to correlate the presence of these immunoreactivities with relative age of the taste cells. Primary gustatory nerve terminals within the CNS also will be tested for immunoreactivity to these growth-related markers. In addition, the ultrastructure of primary gustatory terminals within the CNS will be examined in a continuation of our studies attempting to relate terminal morphology to chemospecificity. Finally, the olfactory system will be examined for 5B4 and GAP-43 immunoreactivities since olfactory neurons continue to be generated and grow into adulthood. Biochemical maturation of the receptors will be studied by means of immunoreactivity to olfactory marker protein, and the growth-related neural antigens. In addition, collaborative studies will continue in terms of determination of the taste bud neurotransmitter, identification of neuropeptides in human biopsy material, and delineation of nerve fibers within taste buds.

味觉和嗅觉的化学感应细胞的周转率 系统需要大量的神经元生长和重塑 外周和中枢神经中的突触连接 系统。 该项目中的实验旨在测试 这些系统中神经元的生长和可塑性程度 使用针对的免疫细胞化学探针 神经元与生长有关的抗原。 这些实验会 检查原发性化学感应神经和受体细胞 味蕾是对抗体免疫反应性的证据 针对神经元膜蛋白5B4和间隙 - 43，两者在成长中的神经元中都具有很高的水平，但 在突触稳定的神经元中减少。 味蕾细胞 他们本身将被测试是否存在GAP-43，因为有些 味道细胞表达5B4免疫反应性。 胸氨酸的tri胺 技术将用于将这些存在的存在相关联 具有相对年龄的味觉细胞的免疫反应性。 基本的 中枢神经系统内的味道神经终端也将进行测试 对这些与生长有关的标记的免疫反应性。 此外， 中枢神经系统内主要阵风的超微结构 将在我们的研究继续进行研究中检查 将末端形态与化学特异性相关。 最后， 将检查5B4和GAP-43的嗅觉系统 免疫反应性由于嗅觉神经元继续是 产生并成年。 生化成熟 将通过免疫反应性研究受体 嗅觉标记蛋白和与生长有关的神经抗原。 此外，协作研究将继续 味蕾神经递质的确定，鉴定 人类活检材料中的神经肽和神经的描述 味蕾内的纤维。