MOLECULAR STUDIES OF HUMAN BONE ALKALINE PHOSPHATASE

人骨碱性磷酸酶的分子研究

• 批准号: 3088515
• 负责人: JOSEPH H ZERNIK
• 金额: $ 7.09万
• 依托单位: UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-07-01 至 1992-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3088515
• 关键词: alkaline phosphatase; genetic manipulation; human tissue; molecular cloning; normal ossification

Alkaline phosphatase is an enzyme which is typically expressed in cells of calcified tissues: osteoblasts, odontoblasts and hypertrophic chondrocytes. Its exact function is unknown; however, in bone it may play a role in mineralization. In the human genetic disease infantile hypophosphatasia, the deficiency in this enzyme is linked to lack of mineralization. Our initial attempts centered on cloning the human gene for bone alkaline phosphatase. However, eventually we isolated genomic clones comprising various parts of the corresponding rat gene. Two of these genomic clones, representing the beginning and the end of this gene are in advance stage of characterization. In addition, we initiated studies of the molecular regulation of alkaline phosphatase expression: using recombinant DNA techniques we produce genetic constants containing various parts of this gene and introduce them into rat bone cells, in order to study their function.

碱性磷酸酶是一种酶，通常在 钙化组织的细胞：成骨细胞，牙本质细胞和 肥厚的软骨细胞。 它的确切功能未知； 但是，在骨头中，它可能在矿化中起作用。 在 人遗传病婴儿次磷酸，缺乏症 在这种酶中，与缺乏矿化有关。 我们的最初尝试以克隆人类基因的骨头为中心 碱性磷酸酶。 但是，最终我们分离了基因组 克隆包含相应大鼠基因的各个部分。 这些基因组克隆中的两个，代表起点和 该基因的结尾处于表征的阶段。 此外，我们启动了有关分子调节的研究 碱性磷酸酶表达：使用重组DNA 我们生产的遗传常数包含各个部分 该基因并将其引入大鼠骨细胞，以便 研究他们的功能。