NUTRITIONAL INFLUENCE ON HYPOTHALAMIC CONTROL

营养对下丘脑控制的影响

• 批准号: 3087015
• 负责人: MAHMOUD N GHAZZI
• 金额: $ 7.59万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-09-30 至 1993-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3087015
• 关键词: evoked potentials; growth media; hormone regulation /control mechanism; hypothalamus; isolation perfusion; laboratory rat; menstrual cycle disorder; monitoring device; nutrition; nutrition related tag; oxygen tension; radioimmunoassay; thermometry; tissue /cell culture

The overall goal of the proposed study is to determine if blood- borne signals (insulin, glucose, amino-acids, fatty acids and ketones) act directly on hypothalamic tissue to modulate the release of LHRH In hypothalamic explants during perifusion. Such effect could explain the pathophysiology underlying clinical syndromes observed in anorexic and athletic women associated with altered reproductive function. This objective will be approached through use of a unique, custom-designed, computer- controlled perifusion system developed at The University of Michigan, and the use of a multi-sensor micro-probe for online monitoring of tissue's electrical multi-unit activity, temperature and oxygen tension. This system allows the user to provide a defined, yet dynamically-changing, pattern of nutrients and stimuli to cultured cells as well as balancing oxygen tension and flow rate to reduce central hypoxia and minimize peripheral oxygen toxicity in the explant. Hypothalamic tissue will be obtained from mature, ovariectomized female rats, placed in the perifusion system, and periodically exposed to higher or lower concentrations of the desired signal. In particular, attempts will be made to determine the effect on the oscillatory activity of the hypothalamic pulse generator by monitoring the following response parameters: (1) the tissue's electrical activity (evoked potentials) measured at multiple sites in the medial basal-preoptic area of the hypothalamus and examined for periodic changes in frequency of electrical activity, transition time from one frequency to another and signal amplitude (using potentiometry); and (2) LHRH release to the surrounding media, quantified by radioimmunoassay (RIA).

拟议研究的总体目标是确定是否血液 传播信号（胰岛素，葡萄糖，氨基酸，脂肪酸和 酮）直接作用于下丘脑组织以调节 在促进过程中，下丘脑外植体中LHRH的释放。 这样的 效应可以解释临床的病理生理学 在厌食和运动女性中观察到的综合征 具有改变的生殖功能。 这个目标将是 通过使用独特的，定制的，计算机 - 受控的枢纽系统在大学开发 密歇根州，以及在线使用多传感器的微型探针 监测组织的电气多单元活性，温度 和氧气张力。 该系统允许用户提供 定义但动态变化的营养模式和 刺激对培养的细胞以及平衡氧张力和平衡 流速以减少中央缺氧并最小化外周 外植体中的氧毒性。 下丘脑组织将是 从成熟的卵巢切除雌性大鼠获得 促进系统，并定期暴露于更高或更低 所需信号的浓度。 特别是，尝试 确定对对振荡活性的影响 下丘脑脉冲发生器通过监测以下 响应参数：（1）组织的电活动（诱发 电位）在内侧基元中的多个位点测量 下丘脑区域，并检查了周期性的变化 电活动的频率，从一个 频率到另一个和信号振幅（使用电位计量法）； （2）LHRH释放到周围媒体，由 放射免疫分析（RIA）。