A GENETIC APPROACH TO VERTEBRATE NEURAL DEVELOPMENT

脊椎动物神经发育的遗传方法

• 批准号: 3088895
• 负责人: Carl Fulwiler
• 金额: $ 9.36万
• 依托单位: HARVARD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-08-01 至 1995-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3088895
• 关键词: cell cell interaction; developmental genetics; developmental neurobiology; fish; fresh water environment; gene expression; gene mutation; genetic manipulation; genetic regulation; genetically modified animals; growth /development; molecular biology; retina; transfection; visual pathways

My goal is to understand the genetic control of neural organization in vertebrates. I have initiated a new approach to this problem using the retina as a model for patten formation in the central nervous system: we will isolate specific mutations in the zebrafish retina and use phenotype analysis and molecular biology to characterize genes controlling retinal organization. The zebrafish provides an opportunity to develop an extremely fast-moving genetic system to explore developmental genes. Its primary advantage is the ability to exploit parthenogenetic development for genetics. The procedures I describe will make it possible to use genetic methods on a large scale to isolate novel vertebrate genes involved in specific functions. Methods will be developed for screening thousands of transgenic lines to identify retinal genes by insertional mutation and by lacZ expression. Mutants will be used to study the cellular interactions involved in assembling a retina and the molecules that mediate these interactions.

我的目标是了解神经组织的遗传控制 脊椎动物。我已经使用 视网膜作为中枢神经系统中Patten形成的模型：我们 将在斑马鱼视网膜中隔离特定突变并使用表型 分析和分子生物学以表征控制视网膜的基因 组织。 斑马鱼提供了开发极快发展的机会 探索发育基因的遗传系统。它的主要优势是 能够利用遗传学的单骨发育发展。 我描述的过程将使在某人上使用遗传学方法 大规模分离出参与特定的新型脊椎动物基因 功能。将开发用于筛选数千种转基因的方法 通过插入突变和LACZ识别视网膜基因的线 表达。突变体将用于研究细胞相互作用 参与组装视网膜和介导的分子 互动。