IN SITU HYBRIDIZATION STUDY OF MYELOID GENE EXPRESSION

骨髓基因表达的原位杂交研究

• 批准号: 3085717
• 负责人: ALAN G ROSMARIN
• 金额: $ 7.43万
• 依托单位: MIRIAM HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-07-15 至 1992-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3085717
• 关键词: acute leukemia; bone marrow; cell differentiation; cell population study; chromosome translocation; chronic leukemia; chronic myelogenous leukemia; complementary DNA; computer data analysis; gene expression; gene induction /repression; genetic library; genetic manipulation; genetic transcription; histochemistry /cytochemistry; human subject; in situ hybridization; messenger RNA; molecular cloning; myeloid stem cell; neoplasm /cancer chemotherapy; neoplasm /cancer diagnosis; neoplasm /cancer genetics; neoplasm /cancer remission /regression; neoplastic cell; nucleic acid probes; nucleic acid sequence; oncogenes; protein sequence; radiotracer; surface antigens; tissue /cell culture; tumor antigens

Oncogenes appear to play important roles in the processes of cellular proliferation and differentiation. Technical problems have limited the study of their expression by normal myeloid cells. The techniques of in situ hybridization allow the analysis of gene expression by individual cells. We propose to apply the techniques of in situ hybridization to normal and leukemic myeloid cells. This will permit comparison of the expression of oncogenes by normal myeloid cells with the expression by leukemic cells, and a correlation of leukemic oncogene expression with their morphologic, histochemical, and chromosomal characteristics. A cDNA library for myeloid cell differentiation will be created and from this library a gene whose expression is increased, and a gene whose expression is decrease during myeloid differentiation will be cloned. These genes will be characterized and sequenced, and the amino acid sequence and control regions will be compared to those of other known proteins. These genes will be used to create probes for in situ hybridization, and the expression of these genes by normal myeloid and leukemic cells will be studied.

癌基因在过程中似乎起着重要作用 细胞增殖和分化。 技术问题 通过正常髓样限制了对表达的研究 细胞。 原位杂交的技术允许分析 单个细胞的基因表达。 我们建议应用 原位杂交与正常和白血病的技术 髓样细胞。 这将允许比较 正常髓样细胞的癌基因与表达 白血病细胞和白血病表达的相关性 具有形态学，组织化学和染色体 特征。 将创建用于髓样细胞分化的cDNA库 从这个库中，一个表达增加的基因，一个 基因在髓样分化过程中的表达降低 将被克隆。 这些基因将被表征和测序， 并且将比较氨基酸序列和对照区域 到其他已知蛋白质的蛋白质。 这些基因将用于 为原位杂交创建探针，这些探针的表达 正常髓样细胞和白血病细胞的基因将被研究。