CELL REGULATION OF GROWTH PLATE MATRIX MINERALIZATION

生长板基质矿化的细胞调节

• 批准号: 3079029
• 负责人: Joseph P. Iannotti
• 金额: $ 7.77万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-07-01 至 1987-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3079029
• 关键词: acid base balance; atomic absorption spectrometry; calcification; cartilage metabolism; cell growth regulation; cell membrane; chondrocytes; endoplasmic reticulum; extracellular matrix; fluorescent dye /probe; homeostasis; hormone regulation /control mechanism; mitochondria; normal ossification; oxygen tension; phosphorus metabolism; vitamin D; vitamin metabolism

It is the purpose of this research proposal to define the mechanisms that regulate intracellular calcium homeostasis in the mammalian growth plate chondrocyte. We will study the effects of parathormone somatomedin, the Vit D matabolites and the local microenvironmental concentration of PO4=, O2 and pH on growth plate chondrocyte calcium transport and homeostasis. It is intended that this information will lead to a better understanding of normal growth plate matrix mineralization and the role of the chondrocyte and their mitochondria in this process. The candidate has completed the equivalent of two years of research at the postdoctoral level. One year has been on a full time basis in the area of growth plate physiology and mitochondrial calcium transport. The other year has been on a part-time basis during the last three years of clinical training in Orthopaedic Surgery. This latter research has been in growth plate chondrocyte physiology and in the area of in vivo scar inhibition using cis hydroxyproline. The University of Pennsylvania is an ideal environment for training in the area of chondrocyte physiology and intracellular calcium homeostasis.

本研究计划的目的是定义以下机制： 调节哺乳动物生长板的细胞内钙稳态 软骨细胞。 我们将研究甲状旁腺激素生长调节素的作用， 维生素D代谢物和局部微环境浓度PO4=， O2 和 pH 对生长板软骨细胞钙转运和稳态的影响。 这些信息旨在使人们更好地了解 正常生长板基质矿化和软骨细胞的作用 以及他们在此过程中的线粒体。 候选人已完成相当于两年的研究 博士后级别。 在以下领域全职工作了一年 生长板生理学和线粒体钙转运。 另一个 在临床的过去三年中，一年一直是兼职的 骨科手术培训。 后一项研究一直在增长 板软骨细胞生理学和体内疤痕抑制领域 使用顺式羟脯氨酸。 宾夕法尼亚大学是一个理想的培训环境 软骨细胞生理学和细胞内钙稳态领域。

项目成果

Subcellular regulation of the ionized calcium pool in isolated growth-plate chondrocytes.

分离生长板软骨细胞中离子钙库的亚细胞调节。

• 发表时间: 1989
• 期刊: Clinical orthopaedics and related research
• 影响因子: 4.2
• 作者: Iannotti,JP;Brighton,CT;Stambough,JE
• 通讯作者: Stambough,JE

Cytosolic calcium concentration in bovine growth plate chondrocytes.

牛生长板软骨细胞中的胞质钙浓度。

• DOI: 10.1002/jor.1100050115
• 发表时间: 1987
• 期刊: Journal of orthopaedic research : official publication of the Orthopaedic Research Society
• 作者: Star,AM;Iannotti,JP;Brighton,CT;Armstrong,PF
• 通讯作者: Armstrong,PF

Molecular cloning and sequencing of a rat preprogastrin complementary deoxyribonucleic acid.

大鼠前胃泌素前体互补脱氧核糖核酸的分子克隆和测序。

• DOI: 10.1210/mend-1-4-306
• 发表时间: 1987
• 期刊: Molecular endocrinology (Baltimore, Md.)
• 作者: Fuller,PJ;Stone,DL;Brand,SJ
• 通讯作者: Brand,SJ

Differential gastrin gene expression in rat gastrointestinal tract and pancreas during neonatal development.

新生儿发育过程中大鼠胃肠道和胰腺中胃泌素基因的差异表达。

• 发表时间: 1988
• 期刊: The Journal of biological chemistry
• 作者: Brand,SJ;Fuller,PJ
• 通讯作者: Fuller,PJ