REGULATION AND DEVELOPMENT OF RENAL ACID-BASE TRANSPORT

肾酸碱转运的调节和发展

• 批准号: 3080899
• 负责人: LUC P BRION
• 金额: $ 9万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-07-01 至 1995-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3080899
• 关键词: acid base balance; acidosis; adenosinetriphosphatase; antisense nucleic acid; band 3 protein; carbonate dehydratase; gel electrophoresis; histochemistry /cytochemistry; in situ hybridization; ion transport; kidney function; laboratory rabbit; laboratory rat; messenger RNA; renal tubular transport; tissue /cell culture; video recording system

We will investigate the role of carbonic anhydrase (CA) in the regulation and maturation of urinary acidification and its adaptation to metabolic and respiratory acidosis. First, in cultured cells obtained from inner medullary collecting ducts (IMCD) of either adult or immature rats, we will test the effect of acid loading on the rates of H+ pumping activity and of Cl-/ HCO3 exchange, on carbonic anhydrase (CA) activity and CA messenger RNA (mRNA). Second, we will test in rabbits the response to acid loading of CA activity in cortex, outer medulla and inner medulla and compare that activity to levels of mRNA for CA, band 3 and H+ ATPase. These experiments will reconcile changes in acid-base transport in the kidney with those in mRNA levels. We will compare these responses in vitro and in vivo in immature animals with those in adult animals. These studies will help us to understand why low birth weight infants are more susceptible to acidosis than are adults.

我们将研究碳酸酐酶（CA）在调节中的作用 尿酸化的成熟及其适应代谢和 呼吸酸中毒。首先，在从内部获得的培养细胞中 成人或未成熟大鼠的髓质收集管（IMCD），我们将 测试酸负荷对H+泵送活动速率和 Cl-/ hco3交换，碳酸酐酶（CA）活性和Ca Messenger RNA（mRNA）。其次，我们将在兔子中测试对酸负荷的反应 皮质，外髓质和内髓质中的Ca活动，并比较 CA，Band 3和H+ ATPase的MRNA水平的活性。这些实验 将调和肾脏中酸碱转运的变化 mRNA水平。我们将在体外和体内比较这些反应 未成熟的动物与成年动物中的动物。这些研究将帮助我们 了解为什么低出生体重婴儿更容易受到酸中毒的影响 比成年人。