HUMAN FILARIASIS--LARVAL ANTIGENS IN PROTECTIVE IMMUNITY

人类丝虫病--保护性免疫中的幼虫抗原

• 批准号: 3078954
• 负责人: AMY D KLION
• 金额: $ 3.31万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-08-01 至 1996-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3078954
• 关键词: chimeric proteins; filariasis; genetic library; human subject; immunity; larva; lymphocyte proliferation; nucleic acid sequence; protein sequence

Despite recent advances in vector control and antifilarial drug development, human infection with the filarial parasites, Wuchereria bancrofti (Wb) and Loa Ioa (LI), remains a major cause of morbidity worldwide. While there is no direct proof that protective immunity exists in humans, the presence of populations of long-term residents of endemic areas who are both amicrofilaremic and without clinical manifestations of disease suggests that such immunity may develop in some individuals. These "putatively immune" individuals demonstrate both a cellular and humoral hyperresponsiveness to crude filarial antigens. Since vigorous responses to microfilariae and adult parasites have been associated with the pathophysiology seen in these infections, the infective larval stage appears a more promising target for protective immunity. To circumvent the lack of available parasite material, we plan to screen filarial larval cDNA libraries with sera from "putatively immune" individuals from two filaria-endemic areas in Benin, West Africa. Fusion products from recombinant clones selected in this manner will be assessed for their ability to stimulate T cell proliferation in these same individuals, and clones that remain of interest will be characterized further with respect to their nucleic acid and amino acid sequences, stage specificity and localization in the native parasite.

尽管最近的载体控制和抗抗物药物取得了进步 发育，丝状寄生虫感染的人类感染，Wucheria Bancrofti（WB）和Loa IOA（LI）仍然是发病率的主要原因 全世界。 虽然没有直接证明保护性免疫力 存在于人类中，长期居民的存在 流行病且没有临床的地方性地区 疾病的表现表明，某些免疫力可能会发展 个人。 这些“推定的免疫”个体证明了 对粗丝抗原的细胞和体液高反应性。 由于对微丝虫和成年寄生虫的剧烈反应已经 与这些感染中看到的病理生理学有关 感染性幼体阶段似乎是保护性的更有希望的目标 免疫。 为了避免缺乏可用的寄生虫材料，我们计划 筛选丝状幼虫cDNA库和血清的“预定 来自西非贝宁的两个流行病地区的免疫”个体。 以这种方式选择的重组克隆的融合产品将是 评估了它们刺激T细胞增殖的能力 同一个人和仍然有兴趣的克隆将是 相对于其核酸和氨基酸进一步表征 天然寄生虫中的序列，阶段特异性和定位。