STUDIES OF SENSORY-MOTONEURON CONTACTS

感觉运动神经元接触的研究

• 批准号: 3075070
• 负责人: Lea Ziskind-Conhaim
• 金额: $ 5.36万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-07-01 至 1993-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3075070
• 关键词: afferent nerve; aminoacid analog; aminoacid inhibitor; cell cell interaction; cell differentiation; electrophysiology; embryo /fetus; gene expression; innervation; laboratory rat; motor neurons; muscle cells; neural information processing; neurogenesis; neuropharmacology; organ culture; peripheral nervous system; spinal cord; spinal nerves; spinal reflex; synapses

The objectives of this grant are to study the inductive relationship between developing central and peripheral synapses, and the influence of these synapses on developmental changes in motoneuron properties. To ensure specific and long-term interaction between cells, differentiation of synapses is directed by gene expression as well as by influential signals passing in antidromic and orthodromic directions across the synapse. Detailed information has accumulated describing the function of sensory-motor synapses in the spinal cord of adult mammals, but little is known about their development. Developing central synapses have been intensively studied in invertebrates and non- mammalian vertebrates such as tadpole and chick. This project will focus on studying the electrical, pharmacological and morphological properties of spinal motoneurons of rat embryos, and investigating the roles of developing sensory-motoneuron contacts and peripheral nerve-muscle interactions on these properties. The pattern and time course of development of spinal reflexes and the initial specificity of afferent motoneuron contacts in spinal cord of rat embryos will be examined. These studies will be carried out in vitro using isolated segments of spinal cord. The small size of the embryonic spinal cord allows adequate penetration of oxygen and nutrients into the tissue which remains viable for many hours. Thoracic segments of spinal cord with their adjacent intercostal muscles will be maintained in organ culture for up to 6 days. The advantages of using spinal cord-intercostal muscle explants are: 1) motoneurons are cultured in continuity with the muscles they normally innervate in vivo, and 2) we have previously described in chronological changes that occur during development of intercostal nerve-muscle contacts in vivo and in organ culture, and thus will be able to correlate these changes with the differentiation of motoneurons that innervate them. Maintaining these explants in the controlled environment of organ culture is valuable for determining the signals that influence motoneuron properties and the formation of central neuronal interactions. Understanding the mechanisms involved in specificity of synapses during development may give insight into mechanisms of long- term interactions between cells and reasons for their inability to re-establish these interactions following lesions in the central nervous system.

这笔赠款的目标是研究归纳关系 在发展中央和周围突触之间 这些突触对发展的影响 Motoneuron属性。 确保具体和长期 细胞之间的相互作用，突触的分化是定向的 通过基因表达以及通过传递的影响信号 突触的抗胶质性和正差方向。 详细信息已经累积了描述的功能 成年哺乳动物的脊髓中的感觉运动突触，但 对他们的发展知之甚少。 发展中心 突触已在无脊椎动物和非脊椎动物中进行了深入研究 哺乳动物脊椎动物，例如t和小鸡。 这个项目 将重点研究电气，药理和 大鼠胚胎的脊柱运动神经元的形态学特性， 并研究开发感觉 - 微神经元的作用 这些接触和周围神经肌肉相互作用 特性。 脊柱发展的模式和时间过程 反射和传入运动神经元的初始特异性 将检查大鼠胚胎的脊髓接触。 这些研究将在体外使用孤立的段进行体外进行 脊髓。 胚胎脊髓的小尺寸允许 将氧气和养分足够的渗透到组织中 这仍然可行多个小时。 脊柱的胸部段 绳索及其相邻的肋间肌肉将被保持在 器官文化长达6天。 使用脊柱的优点 绳索间肌肉外植体是：1）运动神经元是 与他们通常支配的肌肉连续培养 体内和2）我们以前在年代变化中描述了 在肋间神经肌肉的发展过程中发生的 在体内和器官文化中接触，因此将能够 将这些变化与运动神经元的分化相关联 这支配了他们。 在受控的 器官文化环境对于确定 影响运动神经元特性和形成的信号 中央神经元相互作用。 了解突触特异性涉及的机制 在开发过程中可能会深入了解长期的机制 细胞之间的术语相互作用和无法的原因 在中央病变后重新建立这些相互作用 神经系统。