FORMATION AND ELIMINATION OF SYNAPSES

突触的形成和消除

• 批准号: 3074675
• 负责人: CHIEN-PING KO
• 金额: $ 5.33万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-01-01 至 1987-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3074675
• 关键词: electrical potential; electrophysiology; freeze etching; fresh water environment; innervation; larva; membrane structure; myofibrils; nerve endings; nervous system regeneration; neural transmission; neuroanatomy; neuromuscular junction; neurotransmitters; pinocytosis; synapses

To understand how the nervous system operates it is important to study structure, function and formation of the synapse. One of the most important structure at the synapse is the site of transmitter release: the active zone. In this proposal the induction of the active zoe at ectopic endplate sites in the frog will be studied with intracellular recording and freeze-fracture electron microscopy. Ectopic junctions will be induced de novo by removing the original endplate zone and implanting original nerves to endplate-free areas. Whether or not the active zone is formed at the ectopic junction will be examined, especially in relation to the postjunctional fold if the latter is also formed. The result will be compared with a previous study on regeneration of the active zone at the original endplate. In addition the morphometry of active zones at ectopic junctions will be analyzed to study if it correlates with the increase in transmitter release shown with intracellular recording. The proposed research will elucidate how the unique organization of the active zone is induced and differentiated. It will also provide a better understanding on correlation between active zone structures and transmitter released efficacy. This basic knowledge on how synapses form and work would throw light on understanding the mechanisms of certain neurological diseases which may involve deficiency in transmitter release.

要了解神经系统如何运作，重要的是要研究 突触的结构、功能和形成。 最有之一 突触的重要结构是递质释放的部位： 活跃区。 在此提议中，在异位诱导活性 zoe 将通过细胞内记录来研究青蛙的终板位点 冷冻断裂电子显微镜。 异位连接将通过去除原始终板从头诱导 区域并将原始神经植入到无终板区域。 无论是否 将检查在异位连接处形成的活性区， 特别是与结后褶皱有关，如果后者也是 形成。 结果将与之前的再生研究进行比较 原始终板处的活动区域。 此外，形态测量 将分析异位连接处的活动区，以研究是否 与发射机释放的增加相关，如图所示 细胞内记录。 拟议的研究将阐明独特的组织如何 活性区被诱导和分化。 也将提供更好的 对活动区结构与发射机之间相关性的理解 释放功效。 关于突触如何形成和工作的基础知识 将有助于理解某些神经系统的机制 可能涉及递质释放缺陷的疾病。