CONNECTIONS OF SUPERFICIAL DORSAL HORN NEURONS

表层背角神经元的连接

• 批准号: 3078206
• 负责人: DAVID DUBUISSON
• 金额: $ 4.29万
• 依托单位: BETH ISRAEL DEACONESS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-05-01 至 1989-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3078206
• 关键词: analgesia; antidromic impulse; axon; dorsal column; histochemistry /cytochemistry; interneurons; microelectrodes; neurochemistry; pain; single cell analysis; spinal cord mapping

This research will examine the connections, functional properties and chemical content of neurons in the dorsal horn of the spinal cord in decerebrate cats and rats. Further understanding of the role of these cells could lead to better means of treating severe pain in humans. Four related projects are proposed: (1) Superficial dorsal horn neurons of laminae 1-2 and deeper neurons of laminae 3-6 which show responses to stimulation of descending pathways relevant to opiate and 'stimulation-produced' analgesia will be characterized by intracellular dye-labelling and by immunohistochemical staining for peptide or enzyme content. (2) Axon trajectories of neurons in laminae 1-2 will be traced by extracellular and intracellular single unit recording and systematic detailed antidromic mapping using stimulation of varying intensities delivered through low-impedance microelectrodes. Representative units will be marked by intracellular staining and examined immunohistochemically. (3) Activity in pairs of dorsal horn neurons, one in laminae 1-3 and the second deeper in laminae 3-6, will be correlated using simultaneous single unit recordings, spike-triggered averaging, and computation of poststimulus time histograms during repetitive stimulation of descending and afferent systems. If consistent patterns of correlation are found, representative units -- one or both of pair -- will be identified by intracellular staining and by immunohistochemical techniques. (4) Receptive fields and activity of individual neurons in the superficial dorsal horn will be examined before, during and after induction of general anesthesis with pentobarbital or alpha-chloralose. Individual units will be followed by prolonged extracellular single unit recordings with repeated mapping of peripheral receptive fields. Representative units will be penetrated and identified by intracellular dye marking.

这项研究将检查连接，功能属性和 神经元的化学含量在脊髓的背角中 脑猫和大鼠。 进一步了解这些作用 细胞可能导致更好地治疗人类剧烈疼痛的方法。 四个 提出了相关项目：（1）浅表背角神经元 拉米娜（Laminae）1-2和层3-6的更深的神经元，该神经元显示出对 刺激与阿片类药物相关的下降途径 “刺激产生的”镇痛的特征是细胞内 染料标签和通过免疫组织化学染色的肽或酶 内容。 （2）层中神经元1-2中神经元的轴突轨迹将通过 细胞外和细胞内单元记录和系统记录 使用不同强度的刺激详细的抗原映射 通过低阻抗的微电极传递。 代表单位会 以细胞内染色和免疫组织化学检查为特征。 （3）双角神经元成对的活性，1-3中的一个 在3-6层中更深的第二层将使用同时单个 单位记录，尖峰触发的平均和刺激后的计算 在重复刺激下降和传入期间的时间直方图 系统。 如果发现一致的相关模式，则代表性 单位 - 一对或两个单位将通过细胞内识别 染色和免疫组织化学技术。 （4）接受场和 表面背角中个体神经元的活性将是 在诱导全身麻醉之前，期间和之后检查 戊巴比妥或α-氯拉糖。 个人单位将随后 长时间的细胞外单个单位记录，重复的映射 外围接受场。 代表单位将被渗透，并且 通过细胞内染料标记鉴定。