GENETIC ANALYSIS OF YEAST TRANSCRIPTION FACTORS

酵母转录因子的遗传分析

• 批准号: 3073549
• 负责人: JAN S. FASSLER
• 金额: $ 6.64万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-09-28 至 1996-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3073549
• 关键词: DNA binding protein; DNA footprinting; Saccharomyces cerevisiae; fungal genetics; fusion gene; gel electrophoresis; gene expression; genetic enhancer element; genetic library; genetic transcription; immunocytochemistry; laboratory rabbit; molecular cloning; mutant; open reading frames; transcription factor; transposon /insertion element

The overall goal of the proposed research is to investigate specific mechanisms of transcription and regulation of transcription in eukaryotes through the characterization of trans-acting transcription factors in yeast. The SPT13 and SPT14 genes of Saccharomyces cerevisiae were identified by mutations that suppress Ty insertion mutations that inhibit adjacent gene expression. Based on both genetic and molecular experiments, SPT13 and SPT14 are candidates to act via the enhancer-like sequence in Ty (transposable) elements. The primary focus of the experiments described is the characterization of SPT13 and SPT14 in order to elucidate their mechanisms of action. In addition, the development of new genetic strategies for the direct and specific identification of genes encoding enhancer-binding factors are proposed.

拟议研究的总体目标是调查具体 真核生物的转录机制和转录调控 通过反式作用转录因子的表征 酵母。 酿酒酵母的SPT13和SPT14基因 通过抑制 Ty 插入的突变来鉴定 邻近基因的表达。 基于遗传和分子 实验中，SPT13 和 SPT14 是通过类增强子发挥作用的候选者 Ty（转座）元件中的序列。 该项目的首要重点是 所描述的实验是按顺序表征 SPT13 和 SPT14 阐明它们的作用机制。 此外，发展 用于直接和特异性识别基因的新遗传策略 提出了编码增强子结合因子。