IRON ACQUISITION BY STAPHYLOCOCCI

葡萄球菌获取铁

• 批准号: 3078885
• 负责人: Loreen A Herwaldt
• 金额: $ 6.39万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-09-01 至 1994-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3078885
• 关键词: Staphylococcus aureus; Staphylococcus epidermidis; Staphylococcus infection; bacterial cytopathogenic effect; chelating agents; iron metabolism; nutrient bioavailability; nutrition related tag; siderophores; transferrin

The growth of staphylococci can be inhibited by aportransferrin, the serum iron-binding protein. Presumably, growth during human infections is also dependent on the ability of staphylococci to acquire iron from the host. Although previous research has examined the mechanisms by which gram-negative organisms acquire iron, little work has been done to elucidate the mechanisms used by gram-positive organisms. For decades, Staphylococcus aureus has been an important cause of community- and hospital-acquired bloodstream infections. Recently Staphylococcus epidermidis has become one of the most common causes of nosocomial bloodstream infections. Yet little is known about the mechanisms by which staphylococci compete with apotransferrin. The long-term objective of the proposal is to develop a comprehensive picture of the mechanisms by which staphylococci acquire iron. Assays of (55)Fe uptake from various substrates, including apotransferrin, will be used to determine which serve as iron sources. Mechanisms used by other bacteria will be evaluated to determine whether they have a role in iron acquisition by staphylococci: bacterial iron chelators (siderophores), bacterial reductases, bacteria- or host-derived organic acids. Mechanisms used by S. aureus will be compared and contrasted with those used by the less virulent staphylococcal species. The Clinical Investigator Award will enable the Principal Investigator (PI) to address critical questions regarding the pathogenesis of staphylococcal infections. The data will give important insights into the differences between infections caused by S. aureus and by less virulent staphylococcal species. The goal is to develop the antibacterial activity of iron deprivation into new approaches for the prevention and treatment of staphylococcal infections. In addition, the PI will gain experience essential for her future career as an independent investigator.

血清脱铁转铁蛋白可抑制葡萄球菌的生长 铁结合蛋白。 据推测，人类感染期间的生长也 取决于葡萄球菌从宿​​主获取铁的能力。 尽管之前的研究已经探讨了其机制 革兰氏阴性生物体获取铁，但在这方面几乎没有做任何工作 阐明革兰氏阳性生物使用的机制。 几十年来， 金黄色葡萄球菌已成为社区和社区感染的重要原因 医院获得性血流感染。 最近葡萄球菌 表皮癣菌已成为院内感染最常见的病因之一 血流感染。 然而人们对其中的机制知之甚少 葡萄球菌与脱铁转铁蛋白竞争。 该提案的长期目标是制定一个全面的 葡萄球菌获取铁的机制图片。 化验 (55)从各种底物（包括脱铁转铁蛋白）中吸收的铁将被 用于确定哪些可作为铁源。 其他机构使用的机制 将评估细菌以确定它们是否对铁有作用 葡萄球菌获取：细菌铁螯合剂（铁载体）， 细菌还原酶、细菌或宿主衍生的有机酸。 机制 金黄色葡萄球菌使用的数据将与金黄色葡萄球菌使用的数据进行比较和对比 毒性较低的葡萄球菌种。 临床研究者奖将使首席研究员 (PI) 解决有关葡萄球菌发病机制的关键问题 感染。 这些数据将为了解差异提供重要见解 由金黄色葡萄球菌和毒性较低的葡萄球菌引起的感染之间 物种。 目标是开发铁的抗菌活性 剥夺预防和治疗的新方法 葡萄球菌感染。 此外，PI 将获得经验 这对她未来作为一名独立调查员的职业生涯至关重要。