LIN-13 AND SPECIFICATION OF CELL FAT

LIN-13 和细胞脂肪的规格

• 批准号: 2169783
• 负责人: CATHY SAVAGE-DUNN
• 金额: $ 2.27万
• 依托单位: RUTGERS, THE STATE UNIV OF N.J.
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-07-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2169783
• 关键词: Caenorhabditis elegans; cell growth regulation; cell transformation; cell type; developmental genetics; gene interaction; growth /development; molecular cloning; nucleic acid sequence; phenotype; suppressor mutations; tissue mosaicism

lin-13 has been identified in screens for mutants defective in C. elegans vulval lineages. It functions to restrict three of the six equipotential vulval precursor cells from undergoing vulval cell fates. Genetic analysis suggests that existing alleles only partially reduce gene function, and that the complete loss of function (null) phenotype may be lethality. So, lin-13 may also have an essential role in development. I propose to study the roles of lin-13 in development using a combination of genetic and molecular analysis. To identify the role lin-13 plays early in development, null alleles will be isolated and characterized. The terminal phenotype of these alleles will be examined, and any cell lineage transformations will be described. Genetic mosaic analysis will be done to determine where in the cell lineage lin-13 activity is needed for viability and for vulval development. Genes that interact with lin-13 will be characterized in two ways: the construction of double mutants with lin-13 and other genes known to function in the vulval pathways; and the isolation and characterization of suppressor mutations. Finally, to begin investigating the molecular mechanisms of lin-13 action, the gene will be cloned and sequenced.

Lin-13已在秀丽隐杆线虫中有缺陷的突变体中鉴定出来 外阴谱系。 它可以限制六个等电位中的三个 外阴前体细胞来自经历外阴细胞命运。 遗传分析 表明现有等位基因仅部分降低基因功能，并且 功能的完全丧失（NULL）表型可能是致命性。 所以， LIN-13在开发中也可能具有重要的作用。 我建议学习 LIN-13在开发中的作用，结合了遗传和 分子分析。 确定lin-13在早期扮演的角色 发育，无效等位基因将被隔离和表征。 终端 这些等位基因的表型将被检查，任何细胞谱系 转换将被描述。 遗传镶嵌分析将进行 确定需要在细胞谱系LIN-13活性中的何处生存能力 以及外阴发育。 与LIN-13相互作用的基因将是 以两种方式表征：用LIN-13构建双突变体 以及已知在外阴途径中起作用的其他基因；和隔离 和抑制突变的表征。 最后，开始 研究LIN-13作用的分子机制，该基因将是 克隆并测序。