YOLK SAC STEM CELL DIFFERENTIATION IN VITRO AND VIVO

卵黄囊干细胞体外和体内分化

• 批准号: 2857944
• 负责人: ROBERT AUERBACH
• 金额: $ 18.61万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-01-01 至 2000-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2857944
• 关键词: MHC class I antigen; MHC class II antigen; cell differentiation; cell population study; cell type; cellular immunity; density gradient ultracentrifugation; early embryonic stage; fibroblast growth factor; flow cytometry; hematopoiesis; hematopoietic stem cells; histocompatibility; host organism interaction; indicator dilution test; laboratory mouse; leukemia inhibitory factor; mixed tissue /cell culture; tissue donors

DESCRIPTION (Adapted from the investigator's abstract): The hypothesis of this application is that in vitro expanded yolk sac hematopoietic stem cells can successfully engraft in histoincompatible hosts in which they then differentiate, thereby establishing immune functions. The specific aims are to: 1) establish conditions that promote self-renewal of yolk sac-derived mouse hematopoietic stem cells in vitro; 2) determine the ability of the expanded yolk sac-derived stem population (a) to seed murine target organs in histocompatible host animals; (b) to generate the various blood cell lineages; and (c) to yield cells capable of functioning in the immune system; and 3) determine whether the expanded yolk sac-derived stem cell population can also seed murine target organs and differentiate in histoincompatible host animals.

描述（改编自调查员的摘要）： 该应用是在体外膨胀的蛋黄囊造血干细胞 可以成功植入历史兼容的宿主中 区分，从而建立免疫功能。 具体目的是 至：1）建立促进蛋黄囊自我更新的条件 小鼠造血干细胞体外； 2）确定 扩大的蛋黄囊源人群（a）到种子鼠靶器官 在组织兼容的宿主动物中； （b）生成各种血细胞 血统； （c）产生能够在免疫中功能功能的细胞 系统; 3）确定扩展的蛋黄囊衍生的干细胞是否 种群还可以播种鼠靶器官并区分 病态兼容的宿主动物。