ALTERATIONS IN THE PITSLRE PROTEIN KINASE IN MELANOMA

黑色素瘤中 PITSLRE 蛋白激酶的变化

• 批准号: 6077090
• 负责人: Mark Anthony Nelson
• 金额: $ 6.2万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-05-01 至 2001-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6077090
• 关键词: apoptosis; cell growth regulation; chromosome aberrations; clone cells; cytogenetics; gene deletion mutation; gene expression; genetic mapping; melanoma; neoplasm /cancer genetics; neoplastic growth; protein kinase

DESCRIPTION: (adapted from the investigator's abstract) The cytogenetic studies in melanoma of this application, indicate that alterations of chromosome 1 are one of the most frequent anomalies of melanoma and that chromosome breaks frequently cluster at band region 1p36. The PITSLRE gene locus maps to band region 1p36 and appears to be involved in apoptotic signaling. Initial studies in their laboratory indicate alterations in PITSLRE gene copies and abnormal PITSLRE protein expression in melanoma. It was hypothesized that alterations in the PITSLRE gene locus on chromosome and 1p36 are involved in melanoma pathogenesis, possibly by disrupting apoptotic signaling pathways and preventing the elimination of tumor cells through normal checkpoint control. To test this hypothesis they proposed the following specific aims: 1) to determine if the PITSLRE genes are deleted in melanoma cell- lines with chromosome 1p alterations; 2) to analyze PITSLRE expression in melanoma cell lines and primary melanoma; 3) to determine the frequency of PITSLRE gene alterations in primary melanoma specimens; and 4) to determine whether PITSLRE genes play a function role in suppressing growth and/or tumorigenicity of melanoma cell lines.

描述：（从研究者的摘要中改编）细胞遗传学 该应用的黑色素瘤研究，表明 1染色体是黑色素瘤最常见的异常之一， 染色体经常在136频段区域聚集。坑 基因基因座图到带区域1p36，似乎参与了 凋亡信号传导。在其实验室中的初步研究表明 Pitslre基因拷贝和异常pitslre蛋白的改变 黑色素瘤的表达。假设在 染色体上的Pitslre基因基因座和1p36参与黑色素瘤 发病机理，可能通过破坏凋亡信号通路和 防止通过正常检查点消除肿瘤细胞 控制。为了检验这一假设，他们提出了以下特定 目的：1）确定在黑色素瘤细胞中是否删除了Pitslre基因 具有1p染色体染色体的线； 2）分析pitslre表达 在黑色素瘤细胞系和原发性黑色素瘤中； 3）确定 原发性黑色素瘤标本中叶裂基因改变的频率；和 4）确定斑点基因是否在 抑制黑色素瘤细胞系的生长和/或肿瘤性。