VASCULAR TOXICITY OF SELECTED ENVIRONMENTAL POLLUTANTS

特定环境污染物的血管毒性

• 批准号: 2152889
• 负责人: Kenneth S. Ramos
• 金额: $ 6.75万
• 依托单位: TEXAS A&M UNIVERSITY HEALTH SCIENCE CTR
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-07-01 至 1997-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2152889
• 关键词: amines; atherosclerosis; autoradiography; benzopyrenes; biological signal transduction; cardiovascular disorder; cell growth regulation; electron microscopy; environmental contamination; environmental toxicology; fluorescent dye /probe; gel electrophoresis; gene expression; laboratory rat; light microscopy; northern blottings; nuclear runoff assay; nucleic acid hybridization; occupational hazard; oncogenes; phosphorylation; quail; radionuclides; southern blotting; tissue /cell culture; vascular smooth muscle; western blottings

My primary goal is to develop a vigorous research program to study cellular and molecular mechanisms involved in the initiation and/or progression vascular disorders upon exposure to environmental/occupational toxins. Although during his first two years at Texas A&M University Dr. Ramos was allowed to focus on research activities, his teaching and administrative commitment will increase significantly to 30-40% of his time in the coming years. A RCDA would allow Dr. Ramos to continue to devote most of his efforts to research and research-related activities. Under such conditions, Dr. Ramos should be able to make significant contributions to the field of toxicology, as well as vascular cell biology. Dr. Ramos is an Associate Professor of Toxicology in the Department of Physiology and Pharmacology, College of Veterinary Medicine at Texas A&M University. He also holds joint appointments in the Department of Medical Physiology, College of Medicine and the Texas Agricultural Experiment Station. Approximately 750 square feet of well-equipped laboratory space with an adjacent office are available to the principal investigator. Additional laboratory and office space are available within the Department of Medical Physiology. The Faculty of Toxicology at Texas A&M University consists of 13 toxicologists within the College of Veterinary Medicine, as well as 27 members from other Colleges within the University System. This group encompasses scientists with a diverse background which share a primary interest in Toxicology. The Department of Medical Physiology consists of 8 faculty members sharing broad interests in the area of vascular cell biology. The natural overlap of these two groups with the research interests of the principal investigator provide a rich scientific environment for the professional development of the applicant. In this application, experiments are proposed to test the hypothesis that allylamine and benzo(a)pyrene induce erratic expression of oncogenes to cause the phenotypic modulation of smooth muscle cells from a contractile to a more synthetic state. Phenotypic expression is defined by morphologic, ultrastructural, biochemical and functional criteria. The status of the phosphoinositide signal transduction system will be evaluated to determine if alterations in turnover are involved in the expression of the synthetic phenotype. Studies will be conducted to determine if erratic expression of cellular oncogenes in vivo and in vitro is associated with chemical treatment. The data generated from these studies can be used to better understand the role that exposure to ubiquitous environmental contaminants plays in the development of atherosclerosis and other pathological changes of the vascular wall.

我的主要目标是制定一个充满活力的研究计划来研究 参与启动和/或 暴露于以下物质后进展血管疾病 环境/职业毒素。 虽然在他的头两年 德克萨斯农工大学拉莫斯博士被允许专注于研究 活动，他的教学和行政承诺将会增加 在接下来的几年里，他的时间将大幅增加到 30-40%。 RCDA 将 让拉莫斯博士继续将大部分精力投入到研究中 和研究相关活动。 在这种情况下，拉莫斯博士 应该能够在该领域做出重大贡献 毒理学以及血管细胞生物学。 拉莫斯博士是一名助理 生理学系毒理学教授 德克萨斯农工大学兽医学院药理学。 他还在医学部担任联合职务 生理学、医学院和德克萨斯农业实验 车站。 约750平方英尺设备齐全的实验室 校长可以使用相邻办公室的空间 研究者。 提供额外的实验室和办公空间 医学生理学系内。 毒理学部 德克萨斯 A&M 大学由学院内 13 名毒理学家组成 兽医学系以及 27 名来自其他学院的成员 大学系统。 这个群体由具有不同背景的科学家组成 背景主要对毒理学感兴趣。 部门 医学生理学系由 8 名教员组成，共享广泛的 对血管细胞生物学领域感兴趣。 自然重叠 这两个小组的主要研究兴趣 研究者为专业人士提供丰富的科学环境 申请人的发展。 在此应用中，实验是 提出检验烯丙胺和苯并（a）芘的假设 诱导癌基因的不稳定表达，导致表型 平滑肌细胞从收缩性到合成性的调节 状态。 表型表达由形态学定义， 超微结构、生化和功能标准。 的状态 磷酸肌醇信号转导系统将被评估 确定营业额的变化是否与表达相关 合成表型。 将进行研究以确定是否 细胞癌基因在体内和体外的不稳定表达 与化学处理有关。 由这些生成的数据 研究可用于更好地了解接触的作用 无处不在的环境污染物在发展中发挥着作用 动脉粥样硬化等血管壁病理改变。