IRELAND SCIENTIFIC PROGRAM

爱尔兰科学计划

• 批准号: 6203317
• 负责人: Chris M. Ireland
• 金额: $ 9.68万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-15 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6203317
• 关键词: CHO cells; DNA damage; DNA replication; Philippines; antineoplastics; aquatic organism; bioassay; biological products; cell cycle; chemical structure function; drug discovery /isolation; drug screening /evaluation; ecosystem; fermentation; high performance liquid chromatography; infrared spectrometry; marine biology; mass spectrometry; microorganism culture; microorganism metabolism; neoplasm /cancer pharmacology; nuclear magnetic resonance spectroscopy; tubulin; ultraviolet spectrometry

The objective of this sub-program of the NCDDG is to discover natural products roduced by marine invertebrate animals and/or associated micro-organisms that display activity in Cyanamid's Oncology Drug Screen (CODS). The specific aims developed to accomplish this goal are described below. I. To collect a variety of marine invertebrate animals (340 species annually) from different geographical locales and ecological niches. The majority of the samples (approximately 300) will be obtained from the Philippines as part of a collaboration with Dr. Edgardo Gomez, Director, UP-MSI. Samples will also be obtained from the shallow water reef, open ocean pelagic and benthic marine communities of Bermuda. 2. Micro-organisms from marine habitats will be isolated and cultured at Lederle Laboratories. The organisms that display activity will be re-fermented and supplies for isolation studies. 3. To perform bioassay guided isolation of active constituents from the extracts of the above mentioned organisms and to provide these metabolites for biological evaluation. 4. To structurally characterize the active constituents from the above mentioned organisms and to determine their mechanism(s) of action to the extent possible. 5. To perform systematic inventories of biodiversity of the various habitats within the Philippine marine ecosystem. These studies will be important for proper management and conservation of the Philippine marine environment.

NCDDG 子计划的目标是发现自然 海洋无脊椎动物和/或相关产品生产的产品 在 Cyanamid 的肿瘤药物中表现出活性的微生物 屏幕（CODS）。为实现这一目标而制定的具体目标是 如下所述。 一、采集多种海洋无脊椎动物（340种） 每年）来自不同的地理区域和生态位。 大部分样本（约 300 个）将来自 作为与 Edgardo Gomez 博士合作的一部分， UP-MSI 总监。样本也将从浅水中获取 百慕大的珊瑚礁、公海中上层和底栖海洋群落。 2. 海洋栖息地微生物的分离和培养 在莱德勒实验室。显示出活性的生物体将是 重新发酵并为分离研究提供原料。 3. 进行生物测定指导下的活性成分分离 上述生物体的提取物并提供这些 用于生物学评价的代谢物。 4. 对上述活性成分进行结构表征 提到的生物体并确定其作用机制 尽可能的程度。 5. 对不同物种的生物多样性进行系统清查 菲律宾海洋生态系统内的栖息地。这些研究将 对于菲律宾的适当管理和保护非常重要 海洋环境。