CONTROL OF SEX SPECIFIC NEURAL DEVELOPMENT AND BEHAVIOR

控制性别特异性神经发育和行为

• 批准号: 2891000
• 负责人: MICHAEL B MCKEOWN
• 金额: $ 27.97万
• 依托单位: SALK INSTITUTE FOR BIOLOGICAL STUDIES
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-01 至 2001-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2891000
• 关键词: Drosophilidae; behavioral /social science research tag; developmental neurobiology; ethology; gender difference; neurogenetics; sex behavior

DESCRIPTION (Adapted from applicant's abstract): This proposal uses a newly discovered gene controlling multiple aspects of sexual behavior and neural development as an entry point to the dissection of the neurological, molecular and genetic mechanisms that generate of a set of complex behaviors in a higher organism. The dissatisfaction (dsf) gene of Drosophila controls appropriate sexual behavior in both males and females including sex partner choice, receptivity to courtship, copulation efficiency and egg laying behavior. It is also necessary for development of identified sex-specific neurons in both males and females. Genetic studies show that dsf defines a novel pathway that controls sexual differentiation of the nervous system but not of the cuticle. A combination of genetic, molecular, behavioral and neurological experiments will be used to investigate the mechanisms behind the generation of male- and female-specific behaviors. The structure and expression pattern of dsf will be used to gain insight into the mechanism by which dsf functions in the regulation of neural development. Studies of the temporal, spatial and sex-specific regulation of dsf function will be performed to understand integration of dsf into the general regulatory mechanisms functioning within the nervous system and into the genetic cascade which controls sexual development. The expression pattern of dsf and the projections of dsf-expressing cells will be mapped as part of the construction of a wiring diagram of the sex-specific nervous system. Various techniques will be used to study the specific defects that occur in both dsf-expressing and dsf-non-expressing cells in the absence of dsf function. A functional map of the sexual nervous system, both as it relates to general male and female behaviors and to the role of dsf, will be generated via the use of the regulatory sequences of dsf and other genes to target expression of potential key regulatory components to limited regions of the nervous system. Such "genetic sex-changes" will be coupled to behavioral analysis of animals whose non-nervous system sexual differentiation is genetically locked in a male or female mode, thus eliminating complications arising from intersexual or abnormal cuticular development. Genetic screens for genes which functionally interact with dsf will be initiated.

描述（改编自申请人的摘要）：该提案使用新的 发现了控制性行为和神经多个方面的基因 发展是神经系统解剖的切入点， 产生一组复杂行为的分子和遗传机制 在更高的生物体中。 果蝇对照的不满（DSF）基因 包括性伴侣在内的男性和女性的适当性行为 选择，对求爱，交配效率和产卵的选择 行为。 也有必要开发已确定的性别特定的 男性和女性的神经元。 遗传研究表明，DSF定义了 控制神经系统的性别差异的新型途径，但 不是角质层。 遗传，分子，行为和 神经系统实验将用于研究背后的机制 男性和女性特异性行为的产生。 结构和 DSF的表达模式将用于通过 DSF在神经发育的调节中起作用。 研究 DSF功能的时间，空间和性别特定的调节将是 执行以了解将DSF整合到一般监管中 神经系统内部并进入遗传的机制 控制性发展的级联。 DSF的表达模式 并且将表达DSF的细胞的投影将作为映射 构建性别特异性神经系统的接线图。 各种技术将用于研究发生在 在不存在DSF的情况下，表达DSF和表达DSF的单元 功能。 性神经系统的功能图，既与之相关 对于一般的男女行为以及DSF的作用，将是 通过使用DSF和其他基因的调节序列生成 潜在的关键调节组件对有限区域的目标表达 神经系统。 这样的“遗传性变更”将与 对非影响系统性行为的动物的行为分析 分化在遗传上锁定在男性或女性模式下，因此 消除双性或异常表皮引起的并发症 发展。 与DSF功能相互作用的基因的遗传筛选 将开始。