SYNAPTIC MATURATION AND VOCAL PLASTICITY

突触成熟和声音可塑性

• 批准号: 2856590
• 负责人: LAURA L STARK
• 金额: $ 3.67万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-01-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2856590
• 关键词: NMDA receptors; behavior test; biophysics; developmental neurobiology; hormone regulation /control mechanism; male castration; neural plasticity; neural transmission; songbirds; testosterone; verbal learning; vocalization

(adapted from the applicant's abstract): A prominent hypothesis in neuroscience is that synaptic plasticity underlies learning. Vocal learning in song birds offers several advantages for addressing this question: Birdsong is a learned behavior that is acquired and practiced during two relatively discreet developmental periods, and the end product of learning is a stable, stereotyped motor act. To examine potential cellular mechanisms of song learning, physiological and behavioral analyses will be combined in individual birds. Comparing biophysical properties of glutamatergic synaptic transmission in zebra finch nucleus RA with a behavior measure of plasticity, the hypothesis will be tested that a reduced contribution of the NMDA receptor mediated component of synaptic transmission causes crystallization. In addition hormones will be used to manipulate the time course of crystallization by castrating juvenile finches (to prolong variability) or implementing testosterone (to accelerate crystallization). If NMDA receptors are important altering the timing of crystallization should concomitantly alter biophysical changes. Finally, subunit-specific NMDA receptor antagonists will be used to test the hypothesis that a switch in subunits causes the decreased contribution of NMDA receptors to synaptic transmission.

（改编自申请人的摘要）： 神经科学是突触可塑性是学习的基础。人声 在歌鸟中学习提供了解决这个问题的几个优势 问题：Birdsong是一种获得和实践的学识渊博的行为 在两个相对谨慎的发展期间，最终产品 学习是一项稳定的定型运动法。检查潜力 歌曲学习，生理和行为的细胞机制 分析将在单个鸟类中合并。 比较谷氨酸能突触传播的生物物理特性 斑马鳍核Ra具有可塑性的行为量度， 假设将测试NMDA受体的贡献减少 突触传播的介导的成分会导致结晶。在 添加激素将用于操纵时间过程 通过cast割少年芬奇（延长可变性）或 实施睾丸激素（加速结晶）。如果NMDA 受体很重要，改变结晶的时间应 同时改变生物物理变化。最后，亚基特异性NMDA 受体拮抗剂将用于测试一个开关的假设 亚基导致NMDA受体对突触的贡献减少 传播。