MINORITY PREDOCTORAL FELLOWSHIP PROGRAM

少数族裔博士前奖学金计划

• 批准号: 2796733
• 负责人: Michelle R Hamlet
• 金额: $ 1.95万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2796733
• 关键词: Drosophilidae; arthropod genetics; biological signal transduction; cell differentiation; cell type; developmental genetics; embryogenesis; gene interaction; mesoderm; myogenesis; protein tyrosine kinase; protein tyrosine phosphatase

Signaling by receptor tyrosine kinases (RTKs) is a mechanism implicated in developmental processes in organisms as distantly related as yeast and humans. Recently, protein tyrosine phosphatases (PTPases) have been implicated in RTK pathways. The Drosophila PTPase Corkscrew (Csw) is among the first PTPases whose gene mutations are allowing genetic analyses of function in vivo and has been functionally conserved through evolution. The experiments described here utilize genetic, cellular and molecular approaches to determine more precisely the role of Csw during embryogenesis. Therefore, this proposal addresses the following: SPECIFIC AIM I. Determine genetic interactions between Csw and RTKs known to function during embryogenesis. Phenotypic analyses support an hypothesis that Csw functions in several RTK pathways during embryogenesis. To determine whether Csw functions in the DER and Btl RTK pathways experiments are designed to reveal genetic interactions between Csw and each of these RTKs. SPECIFIC AIM II. Determine the role of Csw in RTK signalling during myogenesis. Preliminary results reveal a need for Csw for specification of both heart and muscle precursor cells during embryogenesis. Only a subset of muscle cells (and none of the heart cells) require the DER RTK suggesting that an additional RTK functions in mesoderm specification. Experiments are designed to elucidate the in vivo roles of Csw, as well as a candidate RTK, in the specification of these two mesodermally derived cell types.

受体酪氨酸激酶（RTK）的信号传导是一种与 与酵母和 人类。最近，蛋白质酪氨酸磷酸酶（PTPases）已经 与RTK途径有关。 果蝇PTPase开瓶器（CSW）是 在基因突变允许遗传分析的第一批PTPase中 在体内功能，并通过进化在功能上保守。 这里描述的实验利用遗传，细胞和分子 更精确地确定CSW在 胚胎发生。 因此，该提案解决以下内容： 特定目的I.确定CSW与RTK之间的遗传相互作用 已知在胚胎发生过程中起作用。 表型分析支持 假设CSW在多个RTK途径中起作用 胚胎发生。 确定CSW在DER和BTL RTK中是否功能 途径实验旨在揭示遗传相互作用 CSW和这些RTK中的每一个。 具体目标II。 确定CSW在RTK信号传导中的作用 肌发生。 初步结果表明需要CSW进行规范 胚胎发生过程中心脏和肌肉前体细胞的。 只有一个 肌肉细胞的子集（和无心脏细胞）需要DER RTK 表明在中胚层规范中有其他RTK功能。 实验旨在阐明CSW的体内角色以及 候选RTK，在这两个中胚层的规范中 细胞类型。