PATHOGENESIS OF CONJUNCTIVITIS IN THE EYE

眼结膜炎的发病机制

• 批准号: 2882935
• 负责人: Santa Jeremy Ono
• 金额: $ 24.69万
• 依托单位: SCHEPENS EYE RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-03-01 至 2001-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2882935
• 关键词: alleles; allergens; artificial chromosomes; atopy; clinical research; conjunctivitis; gene expression; genetic markers; genotype; human genetic material tag; human subject; hypersensitivity; immunologic skin test; linkage mapping; major histocompatibility complex; molecular cloning; pathologic process; polymerase chain reaction; questionnaires; seasons; single strand conformation polymorphism; southern blotting; subtraction hybridization

DESCRIPTION: Conjunctivitis is the major atopic disease affecting the ocular surface. Indeed, it is one of the most commonly observed diseases in ophthalmic clinics. Patients with the condition are hypersensitive to normally innocuous substances and suffer from symptoms ranging from mild itching and burning, to chemosis, conjunctival hyperemia, lid edema and at its worst, scarring of the ocular surface and permanent visual impairment. New therapeutic agents are desired due to the side affects of current medications, and the failure of these approaches in severe cases. The design of new drugs require a better understanding of the pathophysiology and biogenesis of the disease. Previous data indicate that while the pathophysiology of atopic conjunctivitis resembles that of rhinitis and asthma, there are also important variables that determine whether allergic inflammation will occur in each, or only a subset of these disparate sites in a particular individual. All data indicate that patients with allergic conjunctivitis share the same atopic condition with rhinitics and asthmatics. The patients with elevated serum gE levels, and the Th2 type cytokines are produced from both Tcells and degranulating mast cells and basophils in the conjunctival epithelium and stroma. However, a large portion of skin test positive individuals would only develop an allergic response in a specific mucosae. The PI hypothesizes that there are particular gene products that specifically target the allergic response to the eye in patients with conjunctivitis, just as they are thought to be specific asthma-predisposing genes. She and others have made significant progress in our efforts to identify potential "atopy" associated markers as well as candidate atopic genes, and will systematically assess the contribution of each of those to atopic conjunctivitis in a large population of patients. Recent progress from her group is included in the preliminary data section of the grant. The first phase of linkage studies will focus on selected markers near (or within) candidate atopic genes. This will be followed by a genomic wide scan for novel determinants using microsatellite markers spaced evenly throughout the human gene map. The PI hopes that the identification of gene products involved in the pathogenesis of allergic conjunctivitis will permit the development of novel therapies for those afflicted with the disease and with other inflammatory diseases of the eye.

描述：结膜炎是影响该疾病的主要特征性疾病 眼表面。 确实，它是最常见的疾病之一 眼科诊所。 病情的患者对 通常是无害的物质，症状从轻度 瘙痒和燃烧，化学，结膜充血，盖子水肿和在 它最糟糕的是，眼表面疤痕和永久视觉障碍。 由于电流的侧面影响，需要新的治疗剂 在严重情况下，药物以及这些方法的失败。 这 新药的设计需要更好地了解病理生理学 和疾病的生物发生。 以前的数据表明，虽然 特应性结膜炎的病理生理类似于鼻炎和 哮喘，也有重要变量确定是否过敏 炎症将发生在每个不同位点的部分，或仅发生 在一个特定的个人中。 所有数据均表明过敏患者 结膜炎与鼻液和 哮喘患者。 血清GE水平升高和TH2型患者 细胞因子是由TCELL和脱粒的肥大细胞和 结膜上皮和基质中的嗜碱性粒细胞。 但是，一个大 皮肤测试的一部分阳性个体只会发展出过敏性 特定粘膜的反应。 PI假设有 特定针对过敏反应的特定基因产物 结膜炎患者的眼睛，就像他们认为是 特定哮喘特定基因。 她和其他人发挥了重要意义 我们努力确定潜在的“特应”相关标记为 以及候选基因，并将系统地评估 每个人对大量人群中特应性结膜炎的贡献 患者。 她的小组的最新进展包括在初步中 赠款的数据部分。 链接研究的第一阶段将重点放在 候选基因附近（或内部）的选定标记。 这将是 然后进行基因组广泛的扫描，用于使用微卫星进行新的决定因素 标记在整个人类基因图中均匀间隔。 PI希望 鉴定与过敏性发病机理有关的基因产物 结膜炎将允许为那些人开发新的疗法 患有疾病和其他眼睛的炎症性疾病。