NEUROENDOCRINE MECHANISMS OF SALT APPETITE

盐食欲的神经内分泌机制

• 批准号: 2856797
• 负责人: STEVEN J FLUHARTY
• 金额: $ 21.22万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-02-15 至 2001-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2856797
• 关键词: G protein; angiotensin II; angiotensin receptor; antisense nucleic acid; appetite; autoradiography; brain mapping; corticosteroids; dietary sodium; estrogens; gender difference; hormone regulation /control mechanism; hypovolemia; laboratory rat; neuroendocrine system; neuropeptide receptor; nuclear runoff assay; nutrition related tag; oxytocin; phospholipase C; receptor expression; salt intake; western blottings

DESCRIPTION (Adapted from the Abstract): The primary object of this project is to examine the cellular and molecular properties of neuronal receptors for two neuropeptides, Angiotensin II (AngII) and oxytocin (OT). That each of these peptides play prominent and opposing roles in the central regulation of sodium appetite is now well established: AngII as a major stimulatory peptide and OT as a mediator of inhibition. The proposed experiments are divided into three main sections. In the first section, the Principal Investigator and his associates will continue their study of the regulation of these neuropeptide receptors in brain by adrenal steroid hormones. In the second series of experiments, they will examine further the cellular actions of AngII and OT and determine how these effects are modulated by adrenal steroids. These experiments will include the use of antisense oligonucleotides to interfere, specifically and reversibly, with the expression of protein (i.e., receptors, G-proteins, and enzymes) involved in AngII and OT action in brain to study directly their involvement in sodium appetite. In the final set of experiments, the researchers will explore some of the possible mechanisms for the sexual dimorphism of sodium appetite, ascertaining the relative contributions of excitatory and inhibitory neuropeptides during estrogen modulation of salt ingestion. Collectively, these experiments should provide a fuller understanding of the cellular mechanisms that mediate neuropeptide control of salt appetite, as well as fundamental insights into the neuroendocrinology of behavior.

描述（根据摘要改编）：该项目的主要对象 是检查神经元受体的细胞和分子特性 对于两种神经肽，血管紧张素II（ANGII）和催产素（OT）。 每个 这些肽在中央扮演着重要和相反的角色 钠食性的调节现在已经确定：Angii是主要的 刺激性肽和OT作为抑制剂的介体。 提议 实验分为三个主要部分。 在第一部分中 首席调查员及其同事将继续研究 通过肾上腺类固醇调节脑中这些神经肽受体 激素。 在第二系列实验中，他们将进一步检查 ANGII和OT的细胞作用，并确定这些效果如何 由肾上腺类固醇调节。 这些实验将包括使用 反义寡核苷酸以干扰，具体和可逆 蛋白质的表达（即受体，G蛋白和酶） 参与ANGII和大脑中的OT作用，直接研究其参与 在钠食性中。 在最后一组实验中，研究人员将 探索一些钠性二态性的可能机制 食欲，确定兴奋性的相对贡献 在摄入盐的雌激素调节过程中，抑制性神经肽。 总的来说，这些实验应该对 介导盐食欲的神经肽控制的细胞机制 以及对行为神经内分泌学的基本见解。