FINE STRUCTURE OF TSTT TERMINALS ON VMPO

VMPO 上 TSTT 端子的精细结构

• 批准号: 2472018
• 负责人: ARTHUR D CRAIG
• 金额: $ 15.53万
• 依托单位: ST. JOSEPH'S HOSPITAL AND MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-12-01 至 2001-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2472018
• 关键词: Primates; brain mapping; central neural pathway /tract; computational neuroscience; histology; neural information processing; pain; perception; sensory mechanism; synapses; temperature; thalamic nuclei

DESCRIPTION (Adapted from the Investigator's Abstract): Our understanding of the functional organization of central pathways for pain and temperature sensations depends on anatomical elucidation of the substrates involved. We recently identified a thalamic nucleus in monkeys and humans (VMpo) that we believe is a sensory relay nucleus for specific pain and temperature activity. We will test this hypothesis by examining the synaptic organization of the lamina I trigemino- and spino-thalamic (TSTT) terminations in VMpo in order to determine whether they provide the basis for secure synaptic relay of sensory information. We will build volume-rendered three-dimensional models of single anterogradely-labeled TSTT terminals from serial ultrathin sections, using a methodology that we have applied successfully in the cat thalamus. Though technically difficult and time consuming, this is the only means of fully characterizing the relationships of these terminals with adjacent ultrastructural elements. In reconstructing their synaptology, we will seek to identify triadic arrangements with presynaptic dendrites, which are characteristic of thalamic sensory relay nuclei but which others say are not associated with TSTT terminals in the primate. Further, we will examine the possibilities that the terminations of nociceptive and thermoreceptive lamina I TSTT fibers may be different, as suggested by prior work in the cat, and that lamina I terminations in the ventral posterior n. (VP) may differ from those in VMpo. These studies will improve our understanding of the thalamic substrates underlying the processing of pain and temperature information, and they will offer insight into the synaptic basis for the sensations of pain and cold that can be produced by stimulation of this region in the human thalamus.

描述（改编自研究者的摘要）：我们的理解 疼痛和温度中枢通路的功能组织 感觉取决于所涉及基质的解剖学阐明。 我们 最近在猴子和人类中发现了丘脑核（VMpo），我们 相信是特定疼痛和温度的感觉中继核 活动。 我们将通过检查突触来检验这个假设 三叉神经和脊髓丘脑 I 层的组织 (TSTT) VMpo 中的终止以确定它们是否提供基础 用于感觉信息的安全突触中继。 我们将建设 单个顺行标记的体绘制三维模型 TSTT 终端采用串行超薄切片，使用我们的方法 已成功应用于猫丘脑。 虽然技术上有难度 并且耗时，这是充分表征的唯一方法 这些终端与相邻超微结构元素的关系。 在 重建他们的突触，我们将寻求识别三元组 突触前树突的排列，其特征是 丘脑感觉中继核，但其他人说与丘脑感觉中继核无关 灵长类动物的 TSTT 终端。 此外，我们将研究可能性 伤害感受和温度感受层 I TSTT 的终止 正如之前在猫身上所做的工作所表明的那样，纤维可能会有所不同，并且 I 层终止于腹侧后部 n. (VP) 可能与那些不同 在VMpo中。 这些研究将增进我们对丘脑的了解 处理疼痛和温度信息的基质， 他们将深入了解感觉的突触基础 刺激该区域可产生疼痛和寒冷 人类丘脑。