FINE STRUCTURE OF TSTT TERMINALS ON VMPO

VMPO 上 TSTT 端子的精细结构

• 批准号: 6478348
• 负责人: ARTHUR D CRAIG
• 金额: $ 7.7万
• 依托单位: ST. JOSEPH'S HOSPITAL AND MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-12-01 至 2001-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6478348
• 关键词: Primates; brain mapping; central neural pathway /tract; computational neuroscience; histology; neural information processing; pain; perception; sensory mechanism; synapses; temperature; thalamic nuclei

DESCRIPTION (Adapted from the Investigator's Abstract): Our understanding of the functional organization of central pathways for pain and temperature sensations depends on anatomical elucidation of the substrates involved. We recently identified a thalamic nucleus in monkeys and humans (VMpo) that we believe is a sensory relay nucleus for specific pain and temperature activity. We will test this hypothesis by examining the synaptic organization of the lamina I trigemino- and spino-thalamic (TSTT) terminations in VMpo in order to determine whether they provide the basis for secure synaptic relay of sensory information. We will build volume-rendered three-dimensional models of single anterogradely-labeled TSTT terminals from serial ultrathin sections, using a methodology that we have applied successfully in the cat thalamus. Though technically difficult and time consuming, this is the only means of fully characterizing the relationships of these terminals with adjacent ultrastructural elements. In reconstructing their synaptology, we will seek to identify triadic arrangements with presynaptic dendrites, which are characteristic of thalamic sensory relay nuclei but which others say are not associated with TSTT terminals in the primate. Further, we will examine the possibilities that the terminations of nociceptive and thermoreceptive lamina I TSTT fibers may be different, as suggested by prior work in the cat, and that lamina I terminations in the ventral posterior n. (VP) may differ from those in VMpo. These studies will improve our understanding of the thalamic substrates underlying the processing of pain and temperature information, and they will offer insight into the synaptic basis for the sensations of pain and cold that can be produced by stimulation of this region in the human thalamus.

描述（改编自调查员的摘要）：我们的理解 疼痛和温度的中心途径的功能组织 感觉取决于所涉及的底物的解剖学阐明。 我们 最近确定了猴子和人类（VMPO）中的丘脑核 认为是特定疼痛和温度的感觉继电器核 活动。 我们将通过检查突触来检验这一假设 Lamina I Trigemino-和Spino-Thalamic（TSTT）的组织 VMPO中的终止以确定它们是否提供基础 有关感官信息的安全突触继电器。 我们将建造 单一标记的体积渲染的三维模型 使用我们的方法论，我们 成功地应用于猫丘脑。 尽管在技术上很困难 而且耗时，这是充分表征的唯一手段 这些终端与相邻的超微结构元素的关系。 在 重建其突触学，我们将寻求确定三合会 与突触前树突的安排，这是 丘脑感官继电器核，但其他人说与 灵长类动物中的TSTT码头。 此外，我们将研究可能性 伤害感受和受感受器薄片的终止 纤维可能有所不同，如猫的先前工作所建议的那样， 腹侧后n的薄片I终止。 （VP）可能与这些不同 在VMPO中。 这些研究将提高我们对丘脑的理解 疼痛和温度信息处理的基础 他们将洞悉突触基础的感觉 可以通过刺激该区域而产生的疼痛和寒冷 人丘脑。