TARGETING AND ASSEMBLY OF E COLI OUTER MEMBRANE PROTEINS

大肠杆菌外膜蛋白的靶向和组装

• 批准号: 6018920
• 负责人: RAJEEV MISRA
• 金额: $ 14.47万
• 依托单位: ARIZONA STATE UNIVERSITY-TEMPE CAMPUS
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-08-01 至 2001-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6018920
• 关键词: Escherichia coli; bacterial capsules; bacterial polysaccharides; bacterial proteins; immunoprecipitation; lipopolysaccharides; membrane biogenesis; membrane lipids; membrane permeability; membrane proteins; protein biosynthesis; suppressor mutations

DESCRIPTION: The targeting and assembly of proteins are fundamental biological processes. In many instances, the targeting signal is defined by small liner stretches of sequence such as those present at the amino terminal end of secreted proteins in bacteria and eukaryotes. While these sequences in Escherichia coli assist proteins in exiting the cytoplasm, they do not determine the final location of secreted proteins within the extracytoplasmic compartments: periplasm and outer membrane. Results from this project have emphasized the view that the targeting signal for outer membrane proteins resides within the partially folded intermediates. The folding states of these intermediates are guided by the intrinsic properties of the molecule and its interactions with other cellular components including chaperone proteins and lipopolysaccharide, which is exclusively localized in the outer membrane. Novel genetic approaches outlined in this project will assist in revealing the nature of intragenic information and provide a better understanding of the dynamic interactions between various cellular components during the targeting and assembly of outer membrane proteins. Because interactions between lipidic components and proteins occur in all biological membranes, the results should have broad general interest. OmpF, OmpC and LamB will be used as model proteins. These proteins serve as the receptor for bacteriophages and form channels that allow the diffusion of water soluble solutes. Three-dimensional structures of OmpF and LamB have been resolved which, while permitting a better understanding of the structure-function relationship of these proteins, shed little light on how they reach their destination. The long term goals of this project are to understand the biogenesis of outer membrane proteins and molecular architecture of the outer membrane. These objectives will be achieved through the application of genetic approaches that are feasible and have already yielded important and new insights.

描述：蛋白质的靶向和组装是基本的 生物过程。 在许多情况下，靶向信号由 小衬里的序列延伸，例如存在于氨基的序列 细菌和真核生物中分泌蛋白的末端。 而这些 大肠杆菌中的序列有助于蛋白质退出细胞质，它们是 请勿确定分泌蛋白在 外质隔室：周质和外膜。 该项目的结果强调了目标信号的观点 对于外膜蛋白，部分折叠 中间人。 这些中间体的折叠状态由 该分子的内在特性及其与其他的相互作用 细胞成分，包括伴侣蛋白和脂多糖， 它仅位于外膜。 新遗传 该项目概述的方法将有助于揭示 基因内信息，并更好地理解动态 靶向过程中各种细胞成分之间的相互作用 外膜蛋白的组装。 因为脂质之间的相互作用 成分和蛋白质发生在所有生物膜中，结果 应该有广泛的兴趣。 OMPF，OMPC和LAMB将用作模型蛋白。 这些蛋白质是 噬菌体和形式通道的受体，可以扩散 水溶性溶质。 OMPF和羔羊的三维结构 已经解决了这一问题，同时允许更好地理解 这些蛋白质的结构功能关系，几乎没有阐明 他们到达目的地。 该项目的长期目标是了解 外膜外膜蛋白和外膜的分子结构。 这些目标将通过应用遗传来实现 可行的方法，已经产生了重要和新的方法 见解。