COMBINATORIAL CONTROL OF TRANSCRIPTION IN YEAST

酵母转录的组合控制

• 批准号: 2910124
• 负责人: ANDREW VERSHON
• 金额: $ 24.05万
• 依托单位: RUTGERS, THE STATE UNIV OF N.J.
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-05-01 至 2002-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2910124
• 关键词: DNA binding protein; Saccharomyces cerevisiae; crosslink; fungal genetics; gene expression; gene induction /repression; genetic regulation; genetic transcription; intermolecular interaction; mutant; nuclear magnetic resonance spectroscopy; protein structure function; site directed mutagenesis; transcription factor

DESCRIPTION: (Adapted from the application) Gene expression in eukaryotes is often under the control of multiple nuclear proteins that work in combination to regulate transcription. The combined interactions between proteins often create a new form of regulatory activity that is distinct from either protein alone. The interactions between these proteins dictate which DNA target sites are bound and therefore which sets of genes are regulated. In some cases, thes protein interactions also influence whether a regulatory protein functions as transcriptional activator or repressor. Understanding the mechanism of how these proteins interact in different combinations, and how these interactions determine the activity and specificity of the complex, will provide insight into the regulation of many developmental and cellular processes. In this project three simple regulatory systems in the yeast Saccharomyces cerevisiae will be investigated for their mechanisms of combinatorial control (1) The alpha2 repressor, a homeodomain protein, interacts with the Mcml and a proteins to turn off two different sets of cell type specific-genes in yeast. The Pi will investigate how alpha2 recognizes the DNA on its own, and how the interactions with Mcml and al influence the target specificity of the complex (Specific aim 1). (2) Mcml, a MADS-box protein, interacts with at least five different co-factor to regulate different sets of genes that are required for cellular processes ranging from cell mating type and arginine metabolism to cell-cycle control. The PI will investigate how Mcml binds DNA on its own (Specific aim 2) and interacts with these co-factors to regulate these diverse sets of genes (Specific aim 3). (3) Pho2, a homeodomain protein, interacts with the Pho4, Bas 1 and Swi5 proteins to regulate genes required for phosphate metabolism, amino acid biosynthesis and cell type switching, respectively. The investigator proposes to investigate how Pho2 binds DNA on its own and interacts with these differen co-factors (Specific aim 4). The proteins in these simple regulatory systems are similar in structure and function to proteins found in mammals and plants. The information learned abou the interactions between these different classes of proteins is expected to be relevant to more complex systems in higher eukaryotes and to provide insight into how these proteins function.

描述：（从应用程序改编）真核生物中的基因表达 通常在多种核蛋白的控制之下 组合调节转录。 之间的结合互动 蛋白质通常会产生一种新的调节活动形式，这是不同的 仅来自任何一种蛋白质。 这些蛋白质之间的相互作用决定了 哪些DNA靶位点是绑定的，因此哪一组基因是 受监管。 在某些情况下，这些蛋白相互作用还会影响 调节蛋白充当转录激活剂或阻遏物。 了解这些蛋白质如何在不同的相互作用的机制 组合以及这些相互作用如何决定活动和 该建筑群的特异性将洞悉许多人的调节 发育和细胞过程。 在这个项目中三个简单 酿酒酵母酵母糖疗法的调节系统将是 研究了它们的组合控制机制 （1）α2抑制剂（一种同源域蛋白）与MCML相互作用 一种蛋白质，可以关闭两组不同的细胞类型特异性基因 酵母。 PI将研究Alpha2如何单独识别DNA， 以及与MCML和AL的相互作用如何影响目标特异性 综合体（特定目标1）。 （2）MCML是一种MADS-box蛋白，与至少五个不同的相互作用 辅助因素调节细胞所需的不同基因集 从细胞交配类型和精氨酸代谢到 细胞周期控制。 PI将研究MCML如何单独绑定DNA （特定目的2）并与这些共同因素相互作用以调节这些 各种基因集（特定目标3）。 （3）PHO2，一种同源域蛋白，与pho4，bas 1和swi5相互作用 蛋白质调节磷酸代谢所需的基因，氨基酸 生物合成和细胞类型切换。 调查员 提议研究PHO2如何自行结合DNA并与 这些不同的共同因素（特定目标4）。 这些简单调节系统中的蛋白质在结构和 在哺乳动物和植物中发现的蛋白质功能。 所学的信息 这些不同类别的蛋白质之间的相互作用是 预计将与更高的真核生物中的更复杂的系统相关，并与 提供有关这些蛋白质如何发挥作用的见解。