REGULATION OF REPRODUCTION

繁殖调节

• 批准号: 2857440
• 负责人: SYNTHIA H MELLON
• 金额: $ 27.53万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-08-01 至 2000-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2857440
• 关键词: DNA binding protein; adrenal glands; animal genetic material tag; animal tissue; cyclic AMP; gel mobility shift assay; gene expression; genetic regulation; hormone regulation /control mechanism; laboratory rat; molecular cloning; nucleic acid sequence; phospholipase C; phosphorylation; polymerase chain reaction; site directed mutagenesis; steroid hormone biosynthesis; testis; transcription factor; transfection

Discussion of the proposal re-emphasized the strong points of the research plan. However, there was some concern that the proposed work may not have a particularly high impact. This factor somewhat moderated enthusiasm DESCRIPTION (adapted from the applicant's abstract): This application proposes experiments to examine how steroid hormone synthesis is controlled in different steroidogenic tissues. To accomplish this, the investigators will focus on the regulation of rat P450c17 in a series of steroidogenic cell lines. These studies will use in vitro transfection and gel shift assays. In Aim 1, the investigators will identify nuclear proteins from rodent Leydig tumor cell and adrenocortical cell lines that interact with regulatory elements in the rat P450c17 gene. In Specific Aim 2, they will clone these factors. In Specific Aim 3, the investigators will explore the role of SF-1 phosphorylation in mediating cAMP and/or basal P450c17 gene expression.

对提案的讨论重新强调了研究的强调 计划。 但是，有人担心拟议的工作可能没有 特别高的影响。 这个因素有些温和的热情 描述（改编自申请人的摘要）：此申请 提出的实验检查了类固醇激素的合成如何控制 在不同的类固醇组织中。 为此，调查人员 将重点介绍一系列类固醇生成的大鼠P450C17的调节 细胞系。 这些研究将使用体外转染和凝胶移位 测定。 在AIM 1中，调查人员将确定来自 啮齿动物Leydig肿瘤细胞和肾上腺皮质细胞系 大鼠P450C17基因中的调节元件。 在特定的目标2中，他们将 克隆这些因素。 在特定目标3中，调查人员将探索 SF-1磷酸化在中介营和/或基础P450C17基因中的作用 表达。