MONITORING A SIDS MODEL IN NEONATAL SWINE

监测新生猪 SIDS 模型

• 批准号: 2889052
• 负责人: PHYLLIS M GOOTMAN
• 金额: $ 29.21万
• 依托单位: SUNY DOWNSTATE MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-09-30 至 2003-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2889052
• 关键词: afferent nerve; baroreceptors; blood chemistry; denervation; disease /disorder etiology; disease /disorder model; electrocardiography; electroencephalography; heart electrical activity; heart function; heart innervation; heart rhythm; hypercapnia; hypoxia; infant animal; model design /development; sleep; statistics /biometry; sudden infant death syndrome; swine; ventricular fibrillation; videotape /videodisc

The long-term objective of the proposed research is to evaluate the role of cardiac mechanisms in the etiology of sudden death in the Sudden Infant Death Syndrome (SIDS). Thus this project is directly related to a problem in infant survival and health. The specific aims of the proposed research are designed to test the hypothesis that a developmental anomaly in cardiac innervation could reduce ventricular electrical stability and favor the onset of sudden cardiac death. The research design and surgical methods have been worked out in the previous grant period; newborn swine with surgically induced imbalance in cardiac autonomic innervation will be continuously monitored (24 hrs.) throughout eight postnatal weeks. Those surviving and similarly surgically prepared littermates will be tested (at ages 2,4,6,8 wks) for cardiovascular responses to: (i) hypoxia, (ii) hypercapnia, (iii) stimulation of cardiopulmonary receptors, and (iv) alterations in baroreceptor afferent inputs. Results obtained from study of these possible precipitating factors should reveal the increased susceptibility of the neonate at risk for sudden death. Sleep is the predominant behavioral state of the neonate and ontogeny of EEG sleep in neonates can be monitored by serial EEG recordings. We plan to examine ECG recordings as a function of state determined by VCR tapes of behavior, diaphragmatic EMG and EEG, and correlate these variables against both age and type of denervation. Nonlinear analysis of heart rate variability in infants has revealed an increase in complexity with maturation and our own results included similar findings in piglets. This grant proposal would pursue these findings and the effects of selected cardiac denervation on this complexity, correlating results with sudden death in denervated piglets. Such studies should confirm our working hypothesis regarding the importance of cardiac innervation in the etiology of sudden death in infants. We are, therefore, postulating an abnormality at the effector level, i.e., at the cardiac level, specifically: maturation of the normal innervation of the heart and its reflex control.

拟议研究的长期目标是评估角色 突然死亡病因中的心脏机制 婴儿死亡综合征（SIDS）。 因此，这个项目与 婴儿生存和健康的问题。 特定目标 拟议的研究旨在检验以下假设 心脏神经的发育异常可以减少心室 电稳定性并有利于心脏猝死的发作。 这 研究设计和手术方法已经在 以前的赠款期；新生猪具有手术诱发的失衡 在心脏自主神经中，将连续监测（24 HRS。）在八个后期。 那些幸存下来的 手术准备的同窝仔（2，4,6,8 WKS）将进行测试 心血管对：（i）缺氧，（ii）超脑，（iii） 刺激心肺受体，（iv）改变 压力感受器传入输入。 从这些研究获得的结果 可能的沉淀因素应揭示增加 新生儿的敏感性有猝死的风险。 睡眠是 EEG睡眠的新生儿和个体发育的主要行为状态 在新生儿中，可以通过串行EEG记录来监视。 我们计划 检查ECG记录是由VCR磁带确定的状态的函数 行为，diaphragmatic EMG和EEG，并将这些变量相关联 对抗年龄和类型的神经服务。 心脏的非线性分析 婴儿的速率差异显示出复杂性的增加 成熟和我们自己的结果包括小猪中的类似发现。 该赠款提案将追求这些发现和 在这种复杂性方面选定的心脏神经研究，将结果相关联 被取消的仔猪突然死亡。 这样的研究应确认 我们关于心脏神经重要性的工作假设 在婴儿突然死亡的病因中。 因此，我们是 假设在效应级别，即心脏异常 特别是：心脏正常神经的成熟 及其反射控制。