RNA LOCALIZATION IN XENOPUS OOCYTES

非洲爪蟾卵母细胞中的 RNA 定位

• 批准号: 2853657
• 负责人: Kimberly L. Mowry
• 金额: $ 23.71万
• 依托单位: BROWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-09-01 至 2004-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2853657
• 关键词: DNA binding protein; DNA footprinting; RNA binding protein; SDS polyacrylamide gel electrophoresis; Xenopus oocyte; affinity chromatography; biological signal transduction; cell type; cellular polarity; cytoskeleton; early embryonic stage; gel mobility shift assay; genetic regulatory element; histogenesis; laboratory rabbit; messenger RNA; microinjections; polymerase chain reaction; protein sequence; site directed mutagenesis

DESCRIPTION (Adapted from the abstract): Establishment of polarity in the egg can be viewed as the earliest step in embryonic patterning. Thus, differences in cell fate among the early cleavage cells are a consequence of asymmetric distributions of informational molecules in the egg cytoplasm before fertilization. The basis for such polarity is provided by localized maternal determinants in the form of protein or mRNA. While there is evidence for the existence of localized determinants in many systems, the localization process is still poorly understood. This research proposal addresses this problem by investigating the biochemical mechanisms underlying mRNA localization, using the frog Xenopus laevis as a model. The PI has purified and cloned an essential localization factor, identified other proteins that are potential localization factors, and defined these critical sequences within an RNA localization signal. The following specific aims are proposed: (1) characterization of VgRBP60, the essential localization factor; (2) investigation of the mechanism of mRNA localization; (3) analysis of other RNA-protein interactions mediating localization, and purification and characterization of other protein components of the localization machinery.

描述（改编自摘要）：极性的建立 在鸡蛋中可以看作是胚胎中最早的一步 图案。因此，早期裂解之间的细胞命运差异 细胞是不对称分布的结果 受精前，卵细胞质中的信息分子。 这种极性的基础由局部母体提供 蛋白质或mRNA形式的决定因素。而有 许多人存在局部决定因素的证据 系统，本地化过程仍然知之甚少。这 研究建议通过研究 使用青蛙，mRNA定位的生化机制 Xenopus laevis作为模型。 PI已纯化并克隆 基本定位因子，确定了其他蛋白质 潜在的本地化因素，并定义了这些关键 RNA定位信号中的序列。以下特定 提出了目的：（1）VGRBP60的表征，必需 定位因子； （2）对mRNA机制的研究 本土化; （3）分析其他RNA蛋白质相互作用 介导本地化，净化和表征 定位机械的其他蛋白质成分。