EAND1H TRANSLOCATION--ENERGY CONVERSION/STRUCTURAL STUDY

EAND1H 易位--能量转换/结构研究

• 批准号: 6018612
• 负责人: J. KENT BLASIE
• 金额: $ 22.32万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-08-01 至 2001-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6018612
• 关键词: X ray crystallography; X ray spectrometry; chemical kinetics; cytochrome b; cytochrome c; cytochrome oxidase; electron transport; lipid bilayer membrane; photosynthetic reaction centers; protein structure function

The overall goal of this project is to determine important aspects of the role of intramolecular and intermolecular structure in biological energy conversion. The structural studies proposed here concern the determination of the cylindrically-averaged profile structures if the natural membrane~s~s molecular components within specific bimolecular complexes involved in energy conversion at moderate resolution (about 10 A); the spatial relationships between these components, relative to each other and to the overall profile structure of the membrane; and the spatial relationships among the redox centers associated with these components, relative to each other within these complexes and relative to the overall profile structure of the membrane, with an accuracy of plus/minus1-2A. In addition, analogous structural studies will be extended to the determination of the 3-D structures of three, much less structurally complex, artificial metalloprotein ~maquettes~ designed to mimic key characteristics of such charge- separating redox enzymes. Through a correlation of such structural parameters with the capabilities of these natural and artificial components and/or complexes thereof to exhibit efficient electron transport and the generation of transmembrane electrochemical potentials (and ultimately the synthesis of ATP) in reconstituted, vectorially-oriented single membrane systems, we hope to gain substantial insight into the mechanism of energy coupling in biological membranes.

该项目的总体目标是确定重要方面 分子内和分子间结构的作用 生物能转化。 提出的结构研究 这里关注的是圆柱冲积的确定 轮廓结构如果天然膜〜s分子 参与的特定双分子复合物中 中等分辨率的能量转换（约10 a）；空间 这些组件之间的关系，相对于彼此和 到膜的整体剖面结构；和空间 与这些相关的氧化还原中心之间的关系 组成部分，相对于这些复合物中的彼此， 相对于膜的整体剖面结构， plus/minus1-2a的精度。 另外，类似的结构 研究将扩展到3D结构的确定 在三个，结构复杂，人造金属蛋白的情况下要少得多 〜Maquettes〜设计为模仿这种电荷的关键特征 - 分离氧化还原酶。 通过这种关联 这些自然和能力的结构参数 人造组件及其复合物以表现出效率 电子传输和跨膜的产生 电化学潜力（最终是ATP的合成） 重构的，面向矢量的单膜系统，我们 希望能够深入了解能源机制 生物膜中的耦合。