STRUCTURE AND FUNCTION OF NUCLEAR RNP PARTICLES

核RNP粒子的结构和功能

基本信息

批准号：
2900631
负责人：
GIDEON DREYFUSS
金额：
$ 15.55万
依托单位：
UNIVERSITY OF PENNSYLVANIA
依托单位国家：
美国
项目类别：
财政年份：
1986
资助国家：
美国
起止时间：
1986-07-01 至 2001-03-31
项目状态：
已结题

项目摘要

Messenger RNAs (mRNAs) are formed in the nuclei of eukaryotic cells by extensive posttranscriptional processing of primary RNA polymerase II transcripts. These transcripts, called heterogeneous nuclear RNAs (hnRNAs) or pre-mRNAs, are associated with specific proteins throughout the time they are in the nucleus. The collective term for the proteins that bind hnRNAs and their nuclear processing products, and which are not stable components of other classes of RNP complexes such as snRNPs, is hnRNP proteins. The hnRNP proteins are avid RNA-binding proteins and the processing of hnRNA in the nucleus takes place in hnRNA-protein (hnRNP) complexes. The significance of hnRNP proteins is that they influence the structure and interactions of hnRNAs and their processing into mRNAs. As several hnRNP proteins shuttle between the nucleus and the cytoplasm, they may also have novel functions in the cytoplasm as well as in nucleo- cytoplasmic transport of mRNAs. The major hnRNP proteins are among the most abundant proteins in the nucleus and hnRNP complexes are thus also of interest because they are major nuclear structures. In order to understand how the post-transcriptional pathway of gene expression operates in the cell, we investigate the structure, function, and localization of the hnRNP proteins. Though much progress has been made in these areas over the past several years, a great deal of fundamental importance remains to be learned. The focus of the research proposed in this grant application is on the further molecular characterization of the hnRNP proteins, including determination of their sequences, structures, RNA-binding activities, protein-protein interactions, assembly into complexes, biochemical activities, and functions in mRNA biogenesis.

信使 RNA (mRNA) 在真核细胞的细胞核中形成初级 RNA 聚合酶 II 的广泛转录后加工成绩单。这些转录本称为异源核 RNA (hnRNA) 或前 mRNA，始终与特定蛋白质相关它们在细胞核中。结合的蛋白质的统称 hnRNA 及其核加工产物，不稳定其他类别 RNP 复合物（例如 snRNP）的组成部分是 hnRNP 蛋白质。 hnRNP 蛋白是狂热的 RNA 结合蛋白，核内 hnRNA 的加工发生在 hnRNA 蛋白 (hnRNP) 中复合物。 hnRNP 蛋白的重要性在于它们影响 hnRNA 的结构和相互作用及其加工成 mRNA。作为一些 hnRNP 蛋白在细胞核和细胞质之间穿梭，它们也可能在细胞质和核中具有新的功能 mRNA 的细胞质转运。主要的 hnRNP 蛋白包括因此，细胞核和 hnRNP 复合物中最丰富的蛋白质也是人们之所以感兴趣，是因为它们是主要的核结构。为了了解基因表达的转录后途径在细胞中运作，我们研究其结构、功能和 hnRNP 蛋白的定位。尽管已经取得了很大进展这些领域在过去几年中取得了很多基础性成果重要性仍有待了解。提出的研究重点该拨款申请是关于进一步的分子表征 hnRNP 蛋白质，包括其序列、结构的测定， RNA 结合活性、蛋白质-蛋白质相互作用、组装成 mRNA 生物合成中的复合物、生化活性和功能。