NEURAL CONTROL OF SEA URCHIN SPINE LIGAMENTS

海胆脊柱韧带的神经控制

• 批准号: 6273598
• 负责人: JOSE DEL CASTILLO
• 金额: $ 1.03万
• 依托单位: UNIVERSITY OF PUERTO RICO MED SCIENCES
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 1999-07-27
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6273598
• 关键词: acetylcholine; actins; alternatives to animals in research; biomechanics; collagen; crosslink; electron microscopy; histochemistry /cytochemistry; immunofluorescence technique; ligaments; microfilaments; muscle contraction; muscle pharmacology; neuromuscular function; neuropharmacology; neuroregulation; neurotransmitters; proteoglycan; sea urchins

The main goal of the proposed research is to elucidate the nature and mechanism of the changes in the physical properties of the collagenous articular capsule, or ligament, that surrounds the spine-test joint of the primary spines of the sea urchin Eucidaris tribuloides. Under the influence of the nervous system the consistency of this ligament can change rapidly and reversibly between two extreme conditions: soft, i.e. extensible, and stiff, or inextensible. Acetylcholine (ACh) consistently stiffens the ligament and may be regarded as the putative catch-inducing neurotransmitter. Pharmacological techniques will be used to search for neurotransmitters or drugs which may induce simple softening and to describe their interactions with ACh. Electron microscopy of conventional preparations and of material stained with cuprolinic blue will be used to characterize cross-links between collagen fibrils and columns and to detect possible alterations in the arrangement of the proteoglycans associated with changes in mechanical properties. In addition, histochemical techniques, using fluorescent phalloidin, anti- actin antibodies and the decoration of actin filaments with heavy meromyosin, will be employed to test the possible presence of actin filaments in the ligament matrix.

拟议研究的主要目标是阐明性质和 胶原型物理特性变化的机制 关节胶囊或韧带，围绕着脊柱测试关节 海胆欧基氏菌的主要刺。 在 神经系统的影响，这种韧带的一致性可以 在两个极端条件之间迅速和可逆地改变：柔软，即 可扩展，僵硬或不可延迟。 乙酰胆碱（ACH）一致 韧带僵硬，可能被视为推定的捕获 神经递质。 药理技术将用于搜索 神经递质或药物可能会引起简单的软化和 描述他们与ACH的互动。 电子显微镜 常规制剂和用杯形蓝色染色的材料 将用于表征胶原原纤维之间的交联 列并检测到排列的可能改变 与机械性能变化相关的蛋白聚糖。 在 另外，使用荧光胶状素，抗 - 肌动蛋白抗体和肌动蛋白丝的装饰 骨膜素将用于测试肌动蛋白的可能存在 韧带矩阵中的细丝。