OPIOID INVOLVEMENT IN NEUROENDOCRINE REGULATION

阿片类药物参与神经内分泌调节

• 批准号: 6270026
• 负责人: EDWIN MARTINEZ
• 金额: $ 11.82万
• 依托单位: COLLEGE AT OLD WESTBURY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-12-01 至 1999-05-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6270026
• 关键词: affinity chromatography; biological signal transduction; calcium channel; communication behavior; drug addiction antagonist; enkephalins; growth hormone releasing hormone; high performance liquid chromatography; intracellular transport; naloxone; neuroendocrine system; neurohormones; neuropeptide receptor; neuropeptides; neurophysiology; neurotransmitter biosynthesis; peptide hormone; receptor binding

The focus of this project is to investigate the interaction between opioid peptides and the hormones that are involved with growth and regeneration in the land crab Gecarcinus lateralis. Intercellular communication mechanisms have been shown to be preserved in evolution, thus the study of G. lateralis.' neuroendocrine system should provide information on how opioids modulate hormonal release in general. The promotion and/or cessation of growth and regeneration in crabs treated with various drugs and drug antagonists (e.g., met-enkephalin, naloxone) will be monitored by periodic measurements of regenerating limbs. Observations on the effects of growth hormones and releasing factors on behavior and behavioral interaction will be made with a Videomex monitoring instrument in conjunction with an Apple IIe computer. HPLC and immunoreactivity experiments will be employed in determination of monoamines and opioid peptides present in the eyestalk ganglia. Binding experiments will be performed to determine the identity of receptors that are involved in opioidhormonal interactions. Results obtained should yield information on intercellular communication and help document the endogenous complexity which will aid in making comparisons to vertebrate biomedical application. The project will also provide a unique model for neuroendocrine behavioral mechanisms with direct applications to mental health. The project will provide student research assistants with the opportunity to be exposed to research and thus be better prepared for graduate and professional training in the biomedical science. They will be immersed in all aspects of the project, from the care of animals, the conduct and assistance of the various experiments, to report writing and presentation at national meetings.

该项目的重点是研究阿片类药物之间的相互作用 与生长和再生有关的肽和激素 在陆地蟹果酱中。细胞间沟通 机制已被证明是在进化中保存的，因此 G. Hursalis。神经内分泌系统应提供有关如何 阿片类药物通常调节激素释放。促销和/或 用各种药物处理的螃蟹的生长和再生停止 和药物拮抗剂（例如Met-enkephalin，纳洛酮）将通过 肢体再生的周期性测量。 观察生长激素和释放因素对 行为和行为互动将通过Videomex监视进行 仪器与Apple IIE计算机结合使用。 HPLC和 免疫反应性实验将用于确定 眼神节中存在的单胺和阿片类肽。结合 将进行实验以确定受体的身份 参与阿片类烟素相互作用。获得的结果应产生 有关细胞间交流的信息，并帮助记录 内源性复杂性将有助于与脊椎动物进行比较 生物医学应用。该项目还将为 神经内分泌行为机制，直接应用于心理 健康。 该项目将为学生研究助理提供机会 接触研究，从而为研究生做好准备 生物医学科学专业培训。他们会沉浸在 该项目的各个方面，来自动物的照顾，行为和 各种实验的协助，报告写作和演示 在国家会议上。