STABLE SYNTHETIC PEROXIDASE FOR VERSATILE ASSAYS

适用于多种检测的稳定合成过氧化物酶

• 批准号: 2844215
• 负责人: SEAN O'MALLEY
• 金额: $ 3.59万
• 依托单位: HAWAII BIOTECH, INC.
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-02-01 至 1998-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2844215
• 关键词: antibody; bioassay; catalyst; chemical conjugate; chemical synthesis; enzyme linked immunosorbent assay; fluoresceins; microcapsule; peroxidases; synthetic enzyme; technology /technique development

A stable, versatile, nonpeptide synthetic peroxidase enzyme mimic will be prepared in the proposed phase I research. The design of the catalyst is based on the well-precedented peroxidase activity of synthetic metalloporphyrins. This catalyst will be readily soluble in organic solvents and will be equipped with functional group handles which will allow aqueous-so lubilizing groups to be attached as well. Conjugation to antibodies and other proteins and to solid phase supports will be demonstrated. The structural aspects of the catalyst have been designed to overcome the limitations of natural peroxidase enzymes by providing better stability, better resistance to inactivation, better resistance to autooxidation, better diffusion characteristics, better accessibility to transduction, and better conjugation features. The improved catalyst will be directly applicable to ELISA and other enzyme-based analytical techniques both as an improved replacement for current reporter peroxidases and as a new innovation to allow organic phase immunoassay development. PROPOSED COMMERCIAL APPLICATION: The proposed catalyst could replace horseradish peroxidase in aqueous ELISA applications. In addition, HBG holds a patent on the use of antibodies in organic phase. Organic phase immunoassay applications utilizing the catalyst would be immediately valuable for detecting environmental contaminants such as polychlorinated aromatics, insecticides, agrochemicals, and others, for which current assay methods are extremely expensive due to labor intensive sample preparation.

稳定的，多才多艺的非肽合成过氧化物酶模拟物将 在拟议的I期研究中准备。 催化剂的设计 基于合成的前所未有的过氧化物酶活性 金属磷脂。 该催化剂将很容易溶于有机 溶剂，并将配备功能组手柄 也可以将水溶性组固定。 共轭 抗体和其他蛋白质以及固相支撑将是 证明。 已经设计了催化剂的结构方面 通过提供的来克服天然过氧化物酶的局限 更好的稳定性，更好地抵抗灭活，更好的抵抗力 为了自动氧化，更好的扩散特征，更好的可访问性 转导和更好的共轭特征。 改进的催化剂将直接适用于ELISA和其他 基于酶的分析技术既可以改进 当前的记者过氧化物酶和作为新创新，以允许有机 相免疫测定开发。 拟议的商业应用：拟议的催化剂可以取代 ELISA水溶液中的辣根过氧化物酶。另外，HBG 对在有机阶段使用抗体的使用持有专利。 有机阶段 使用催化剂的免疫测定应用将立即 对于检测环境污染物（例如多氯）的宝贵 芳香族剂，杀虫剂，农业化学等，目前 由于劳动密集型样本，测定方法非常昂贵 准备。