MAT BIOREACTOR TO RECOVER UNCULTURABLE ORGANISMS

用于回收不可培养生物的 MAT 生物反应器

• 批准号: 2713007
• 负责人: JOAN D COMBIE
• 金额: $ 9.91万
• 依托单位: MONTANA BIOTECH CORPORATION
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2713007
• 关键词: antiAIDS agent; antineoplastics; bioengineering /biomedical engineering; biological products; biomedical equipment development; bioreactors; drug design /synthesis /production; growth media; method development; molecular biology; nucleic acid sequence; polymerase chain reaction; thermophilic organism; tissue /cell preparation

A novel bioreactor has been designed to recover microorganisms that are considered to be "unculturable." Greater than 99 percent of microorganisms in nature have never been cultured. Conventional bioreactors do not mimic the natural habitat and therefore fail to exploit the full potential of these exceptional microbes. A Thermal Mat Bioreactor duplicates the environmental conditions and support surface requirements of microorganisms isolated from a thermal mat community. Design considerations include adhesion requirements, flow rate, light cycles and removal of toxic byproducts. Two field samples, containing a mixed population of "unculturable" microorganisms isolated from thermal mat communities will be used as inoculum for the mat bioreactor. A diversity of parameters will be tested for their value in generating novel microorganisms. The unique identity of the organisms will be demonstrated by molecular analysis. A mat bioreactor represents an unlimited opportunity to discover biomolecules that will fill the needs of the medical, pharmaceutical, bioremediation and material science industries. PROPOSED COMMERCIAL APPLICATION: Novel, "unculturable" microorganisms cultivated by this system will be of commercial value to the biomedical market for anti-tumor and anti-HIV treatments and other pharmaceuticals. The bioreactor itself will be of tremendous value to other industries that develop important biomolecules from exceptional microorganisms.

设计了一种新型生物反应器来回收微生物 被认为是“没有文化的”。 超过99%的 自然界中的微生物从未被培养过。传统的 生物反应器不模仿自然栖息地，因此无法 充分利用这些特殊微生物的潜力。 导热垫 生物反应器复制环境条件和支撑表面 从热垫群落中分离出的微生物的需求。 设计考虑因素包括附着力要求、流速、光线 循环和有毒副产品的去除。 两个现场样本，包含 分离自“不可培养”微生物的混合群体 热垫群落将用作垫生物反应器的接种物。 将测试各种参数在生成中的价值 新型微生物。 生物体的独特身份将是 通过分子分析证明。 垫式生物反应器代表着无限的发现机会 满足医疗、制药、 生物修复和材料科学行业。 拟议的商业应用： 该系统培养的新型“不可培养”微生物将 对抗肿瘤和抗HIV生物医药市场​​具有商业价值 治疗和其他药物。 生物反应器本身将是 对开发重要生物分子的其他行业具有巨大价值 来自特殊的微生物。