LC INTERFACE FOR ACCELERATOR MASS SPECTROMETRY

用于加速器质谱分析的 LC 接口

• 批准号: 2712958
• 负责人: RUTH E SHEFER
• 金额: $ 10万
• 依托单位: NEWTON SCIENTIFIC, INC.
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-23 至 2001-04-22
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2712958
• 关键词: biomedical equipment development; carbon; carcinogenesis; electrospray ionization mass spectrometry; environmental toxicology; liquid chromatography; mass spectrometry; radionuclides; scintillation counter; solvents; tritium

The goal of this project is the development of a new, ultra-sensitive analytical tool for biomedical research that couples the precision separation capabilities of liquid chromatography (LC) with the detection sensitivity of accelerator mass spectrometry (AMS). This new technique, known as LC-AMS, will promote the study of biological molecules labeled with carbon-14 or tritium. AMS is a powerful tool for the detection of 14C and 3H in environmental toxicology and human carcinogenesis research. For 14C, AMS has a 1000-fold higher sensitivity than liquid scintillation decay counting, allowing the quantitation of attomole (10[-18] mole), or smaller, samples. Presently, the analysis of biological samples using AMS is limited by the requirement for highly specialized sample preparation procedures which are not compatible with standard chromatographs. The goal of Phase I is to develop techniques for the real-time coupling of a LC to the input of an AMS system, leading to the construction and testing of a LC-AMS interface in Phase II. This interface will be coupled to a compact biomedical AMS system developed by NSI in order to demonstrate, for the first time, the detection of labeled molecules from a LC using an AMS. It will also be suitable for use with other existing AMS systems. PROPOSED COMMERCIAL APPLICATIONS: The biomedical LC-AMS system will have widespread commercial applications in toxicology and new drug research. With this instrument, it will be possible to conduct drug metabolism and kinetics studies using three orders-of-magnitude less tracer activity than is possible today. The cost and size of the LC-AMS system will be compatible with installation at major medical research centers. The LC-AMS interface will also have a commercial market as a stand-alone instrument which will allow conversion of any existing AMS system to biomedical use.

该项目的目的是开发一种新的，超敏感的 生物医学研究的分析工具，伴随精度 液相色谱（LC）的分离能力与检测 加速器质谱法（AMS）的敏感性。这项新技术， 称为LC-AMS，将促进标记的生物分子的研究 与碳-14或tritium。 AMS是检测的强大工具 环境毒理学和人类致癌研究中的14C和3H。 对于14C，AMS比液体闪烁的灵敏度高1000倍 衰减计数，允许定量attomole（10 [-18] mole）或 较小的样品。目前，使用AM对生物样品进行分析 受高度专业样品准备的要求限制 与标准色谱不兼容的过程。这 第一阶段的目标是开发用于实时耦合的技术 LC到AMS系统的输入，导致构建和 在II期中测试LC-AMS界面。此界面将耦合 NSI开发的紧凑型生物医学AMS系统 首次证明了从 LC使用AMS。它也适合与其他现有的 AMS系统。 拟议的商业应用： 生物医学LC-AMS系统将具有广泛的商业应用 在毒理学和新药研究中。有了这个乐器，它将是 可以使用三个 标准级的示踪剂活动少于当今的可能性。费用 LC-AMS系统的尺寸将与安装兼容 主要的医学研究中心。 LC-AMS接口也将有一个 商业市场作为独立工具，将允许转换 任何现有的AMS系统用于生物医学使用。