P-GLYCOPROTEIN TRANSPORTER IN CRYPTOSPORIDIUM--AIDS OI

隐孢子虫中的P-糖蛋白转运蛋白--AIDS OI

• 批准号: 2672995
• 负责人: SYLVIA M LEBLANCQ
• 金额: $ 8.53万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-06-01 至 2000-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2672995
• 关键词: AIDS; Cryptosporidium; P glycoprotein; Saccharomyces cerevisiae; cryptosporidiosis; molecular cloning; multidrug resistance; nucleic acid sequence; protein structure function; recombinant proteins

DESCRIPTION (adapted from applicant's abstract) The proposed research focuses on the C. parvum P-glycoprotein homologue (Pgh), Pgh is a member of the ABC class of transporters. Pgh is an important target to study in C. parvum as it is known to be refractory to most drugs tested to date. It is not known whether drug transport is the major contributory factor or actual resistance to all the many drugs tested to date. The specific aims of this research are (1) complete the cloning and sequencing of the C. parvum pgh gene; (2) obtain expression of the Pgh transporter in Saccharomyces cerevisiae in such a way that a functional assay can be developed; and (3) use the yeast system as a functional assay of Pgh to test inhibitor activity.

描述（根据申请人的摘要改编）拟议的研究 PGH专注于C. parvum p-糖蛋白同源物（PGH），PGH是 ABC类型的转运蛋白类。 PGH是研究中研究的重要目标。 众所周知，这是对迄今为止测试的大多数药物的难治性。 这是 不知道药物运输是主要因素还是实际因素 迄今为止对所有测试的许多药物的抵抗力。 这个特定的目的 研究是（1）完成C. parvum PGH的克隆和测序 基因; （2）在糖疗中获得PGH转运蛋白的表达 酿酒酵母以一种可以开发功能测定的方式； （3） 使用酵母系统作为PGH的功能分析来测试抑制剂 活动。