HUT REGION OF RHIZOBIUM FREDII

Fredii 根瘤菌的 HUT 区域

• 批准号: 2665107
• 负责人: ANTHONY J NIEUWKOOP
• 金额: $ 8.98万
• 依托单位: GRAND VALLEY STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-01 至 2002-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2665107
• 关键词: Pseudomonas; Rhizobiaceae; bacterial genetics; beta glucuronidase; enzyme activity; fusion gene; gene mutation; genetic regulatory element; genetic transcription; molecular cloning; nitrogen metabolism; nucleic acid sequence

DESCRIPTION (adapted from investigator's abstract): The long term objectives of this research are to examine at the molecular level the regulation of nitrogen utilization by the gram negative soil microorganism, Rhizobium fredii. This group of bacteria is relatively newly discovered (1982) and although some data have been obtained, complete characterization of their biochemistry has not been undertaken. For these studies the histidine utilization (hut) genes of R. fredii, will be used as a model system to understand how it can utilize poor nitrogen sources. The specific aims of this application are to: (1) Determine the number and the activity of the R. fredii hut enzymes. (2) Identify the number and locations of hut specific transcriptional starts. (3) Begin DNA sequence analysis of putative regulatory sites upstream of the transcriptional start sites. (4) Construct GUS (beta-Glucuronidase) fusions with cloned R. fredii hut DNA for use in regulation studies and the isolation of mutant regulatory elements. This project would successfully integrate biochemical and DNA/molecular approaches to characterize this system and provide additional opportunities for detailed investigations into prokaryotic gene regulation.

描述（改编自研究者的摘要）：长期 这项研究的目的是在分子水平上检查 革兰氏阴性土壤微生物对氮利用的调节， 弗雷迪根瘤菌。 这组细菌是相对较新发现的 （1982）虽然已经获得了一些数据，但完整的表征 他们的生物化学尚未进行。 对于这些研究 R. fredii 的组氨酸利用 (hut) 基因将用作模型 系统来了解它如何利用贫乏的氮源。 该应用程序的具体目标是： (1) 确定数量和 R. fredii hut 酶的活性。 (2) 识别号码并 hu 特定转录起始的位置。 (3) 开始DNA序列 转录起始上游假定调控位点的分析 网站。 (4) 与克隆的弗雷迪氏菌构建 GUS（β-葡萄糖醛酸酶）融合体 hut DNA 用于调控研究和突变调控的分离 元素。 该项目将成功整合生化和 DNA/分子 表征该系统并提供额外机会的方法 对原核基因调控的详细研究。