INNOVATIVE IMPLANTABLE GLUCOSE BIOSENSOR

创新的植入式葡萄糖生物传感器

• 批准号: 2870324
• 负责人: In Han
• 金额: $ 9.86万
• 依托单位: M-BIOTECH, INC.
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-01 至 2000-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2870324
• 关键词: acidity /alkalinity; artificial endocrine pancreas; biomaterial compatibility; biomaterial development /preparation; biomaterial evaluation; biomedical equipment development; biosensor device; enzyme activity; gel; glucose metabolism; glucose oxidase; medical implant science

This proposal describes the design and testing of an innovative implantable glucose sensor using pH-sensitive hydrogels and pressure sensing technology. This system is based on pressure transducers and/or piezoelectric transducers in combination with pH-sensitive hydrogels containing glucose oxidase (GOD). The hydrogels swell in response to decreases in pH due to the presence of pH-sensitive pendant groups in the gel. Decreases in pH within the gel occur in response to increases in the surrounding glucose concentration, because gluconic acid is generated by GOD when glucose and oxygen are present. The pH-sensitive hydrogels are swollen by an amount which is proportional to glucose concentration. The swollen hydrogels will give an elevated pressure within the limited chamber which encloses the gel, which can be detected using miniature pressure sensors of well-tested design. Thus glucose concentrations can be continuously monitored using the combination technologies of hydrogels and pressure sensors. The optimal conditions of pH-sensitive gels such as cross-link density, number and type of pendant groups, GOD level, catalase level, gel thickness, etc. will be determined by in vitro measurements of sensor response time, linearity, and oxygen sensitivity, and used to establish the technical merit and feasibility of pressure-based glucose monitoring in Phase I. PROPOSED COMMERCIAL APPLICATIONS: Implantable glucose biosensors developed herein could be commercially applied as clinical sensors.

该建议描述了使用pH敏感水凝胶和压力传感技术的创新植入式葡萄糖传感器的设计和测试。 该系统基于压力传感器和/或压电传感器，结合含有葡萄糖氧化酶（上帝）的pH敏感水凝胶。由于凝胶中pH敏感的吊坠存在，水凝胶响应于pH值减少。 凝胶内pH的降低是由于周围葡萄糖浓度的增加而发生，因为当存在葡萄糖和氧气时，上帝会产生葡萄糖酸。 pH敏感水凝胶的肿胀量与葡萄糖浓度成正比。肿胀的水凝胶将在有限的腔室内产生升高的压力，该腔室包围了凝胶，可以使用经过良好测试设计的微型压力传感器检测到。因此，可以使用水凝胶和压力传感器的组合技术连续监测葡萄糖浓度。 pH敏感凝胶的最佳条件，例如交联密度，吊坠群的数量和类型，上帝水平，过氧化氢酶水平，凝胶厚度等。将通过体外测量传感器响应时间，线性和氧敏感性的体外测量来确定，并用于在此中建立基于压力的gluc gluc gluc gluc gluc In spepers In的技术优点：在相机上的可行性。用作临床传感器。