STAINING REAGENTS FOR DETECTING AUTOREACTIVE CD4 T CELLS

用于检测自身反应性 CD4 T 细胞的染色试剂

• 批准号: 6211006
• 负责人: Peter R Rhode
• 金额: $ 24.5万
• 依托单位: ALTOR BIOSCIENCE. LLC
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-01 至 2002-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6211006
• 关键词: MHC class II antigen; antibody; chimeric proteins; dyes; helper T lymphocyte; immunoglobulin genes; peptide structure; reagent /indicator; technology /technique development

DESCRIPTION: (Adapted from the applicant's abstract) The proposal describes a plan to generate a staining reagent for antigen specific CD4 T cells by modifying the MHC tetramer technology. Specifically, the investigators will create an antibody like molecules whose variable regions have been replace with single chain MHC Class II/peptide structures that will bind to autoreactive CD4 T cells involved in MS. The valency of the tetramers generated by this group can be easily manipulated using well-characterized techniques established to multimerize antibodies. The investigators anticipate that, like the existing MHC tetramer, this product will be of use to researchers who wish to identify antigen specific T cells during the course of disease and because of its multimerization characterization may prove even more valuable. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE

描述：（改编自申请人的摘要）该提案描述了 计划通过以下方式生成抗原特异性 CD4 T 细胞的染色试剂 修改MHC四聚体技术。具体来说，调查人员将 创建一个类似抗体的分子，其可变区被替换为 将结合自身反应性 CD4 的单链 MHC II 类/肽结构 T 细胞参与多发性硬化症。该组产生的四聚体的价数 可以使用已建立的充分表征的技术轻松操纵 多聚化抗体。调查人员预计，就像现有的 MHC 四聚体，该产品将对希望鉴定的研究人员有用 抗原特异性 T 细胞在疾病过程中并且由于其 多聚化表征可能更有价值。 拟议的商业应用：不可用